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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia phytofirmans 50S ribosomal protein L17 (rplQ) CSB-YP462562BXU
CSB-EP462562BXU
CSB-BP462562BXU
CSB-MP462562BXU
CSB-EP462562BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L30 (rpmD) CSB-YP462563BXU
CSB-EP462563BXU
CSB-BP462563BXU
CSB-MP462563BXU
CSB-EP462563BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L5 (rplE) CSB-YP462564BXU
CSB-EP462564BXU
CSB-BP462564BXU
CSB-MP462564BXU
CSB-EP462564BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S3 (rpsC) CSB-YP462565BXU
CSB-EP462565BXU
CSB-BP462565BXU
CSB-MP462565BXU
CSB-EP462565BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 30S ribosomal protein S7 (rpsG) CSB-YP462566BXU
CSB-EP462566BXU
CSB-BP462566BXU
CSB-MP462566BXU
CSB-EP462566BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans 50S ribosomal protein L11 (rplK) CSB-YP462567BXU
CSB-EP462567BXU
CSB-BP462567BXU
CSB-MP462567BXU
CSB-EP462567BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP462568BXU
CSB-EP462568BXU
CSB-BP462568BXU
CSB-MP462568BXU
CSB-EP462568BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Homoserine kinase (thrB) CSB-YP462569BXU
CSB-EP462569BXU
CSB-BP462569BXU
CSB-MP462569BXU
CSB-EP462569BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Betaine aldehyde dehydrogenase (betB) CSB-YP462570BXU
CSB-EP462570BXU
CSB-BP462570BXU
CSB-MP462570BXU
CSB-EP462570BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans UPF0060 membrane protein Bphyt_4776 (Bphyt_4776), partial CSB-YP462571BXU1
CSB-EP462571BXU1
CSB-BP462571BXU1
CSB-MP462571BXU1
CSB-EP462571BXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP462572DUG
CSB-EP462572DUG
CSB-BP462572DUG
CSB-MP462572DUG
CSB-EP462572DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP462573DUG
CSB-EP462573DUG
CSB-BP462573DUG
CSB-MP462573DUG
CSB-EP462573DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0473 protein CLL_A1177 (CLL_A1177) CSB-YP462574DUG
CSB-EP462574DUG
CSB-BP462574DUG
CSB-MP462574DUG
CSB-EP462574DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UPF0296 protein CLL_A1210 (CLL_A1210) CSB-YP462575DUG
CSB-EP462575DUG
CSB-BP462575DUG
CSB-MP462575DUG
CSB-EP462575DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Glutamate racemase (murI) CSB-YP462576DUG
CSB-EP462576DUG
CSB-BP462576DUG
CSB-MP462576DUG
CSB-EP462576DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP462577DUG
CSB-EP462577DUG
CSB-BP462577DUG
CSB-MP462577DUG
CSB-EP462577DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Proline--tRNA ligase (proS), partial CSB-YP462578DUG
CSB-EP462578DUG
CSB-BP462578DUG
CSB-MP462578DUG
CSB-EP462578DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP462579DUG
CSB-EP462579DUG
CSB-BP462579DUG
CSB-MP462579DUG
CSB-EP462579DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 30S ribosomal protein S19 (rpsS) CSB-YP462580DUG
CSB-EP462580DUG
CSB-BP462580DUG
CSB-MP462580DUG
CSB-EP462580DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 50S ribosomal protein L24 (rplX) CSB-YP462581DUG
CSB-EP462581DUG
CSB-BP462581DUG
CSB-MP462581DUG
CSB-EP462581DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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