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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L28 (rpmB) CSB-YP461161AXI
CSB-EP461161AXI
CSB-BP461161AXI
CSB-MP461161AXI
CSB-EP461161AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-glucosidase 11 (BGLU11) CSB-YP461162DOA
CSB-EP461162DOA
CSB-BP461162DOA
CSB-MP461162DOA
CSB-EP461162DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g17910 (At4g17910), partial CSB-YP461163DOA
CSB-EP461163DOA
CSB-BP461163DOA
CSB-MP461163DOA
CSB-EP461163DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi Translation factor GUF1 homolog, mitochondrial (PKH_070910), partial CSB-YP461164EWU
CSB-EP461164EWU
CSB-BP461164EWU
CSB-MP461164EWU
CSB-EP461164EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi Lipoyl synthase, apicoplast (lipA) CSB-YP461165EWU
CSB-EP461165EWU
CSB-BP461165EWU
CSB-MP461165EWU
CSB-EP461165EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi Protein SEY1 homolog (PKH_133110), partial CSB-YP461166EWU
CSB-EP461166EWU
CSB-BP461166EWU
CSB-MP461166EWU
CSB-EP461166EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Biogenesis of lysosome-related organelles complex 1 subunit CNL1 (CLN1) CSB-YP461167SVP
CSB-EP461167SVP
CSB-BP461167SVP
CSB-MP461167SVP
CSB-EP461167SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATPase GET3 (GET3) CSB-YP461168SVP
CSB-EP461168SVP
CSB-BP461168SVP
CSB-MP461168SVP
CSB-EP461168SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 6 (AIM6) CSB-YP461169SVP
CSB-EP461169SVP
CSB-BP461169SVP
CSB-MP461169SVP
CSB-EP461169SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sensitivity to high expression protein 10 (SHE10), partial CSB-YP461170SVP
CSB-EP461170SVP
CSB-BP461170SVP
CSB-MP461170SVP
CSB-EP461170SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial group I intron splicing factor CCM1 (CCM1), partial CSB-YP461171SVP
CSB-EP461171SVP
CSB-BP461171SVP
CSB-MP461171SVP
CSB-EP461171SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial distribution and morphology protein 12 (MDM12) CSB-YP461172SVP
CSB-EP461172SVP
CSB-BP461172SVP
CSB-MP461172SVP
CSB-EP461172SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Genetic interactor of prohibitins 3, mitochondrial (GEP3), partial CSB-YP461173SVP
CSB-EP461173SVP
CSB-BP461173SVP
CSB-MP461173SVP
CSB-EP461173SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit (SPT14), partial CSB-YP461174SVP1
CSB-EP461174SVP1
CSB-BP461174SVP1
CSB-MP461174SVP1
CSB-EP461174SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Increasing suppression factor 1 (ISF1) CSB-YP461175SVP
CSB-EP461175SVP
CSB-BP461175SVP
CSB-MP461175SVP
CSB-EP461175SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATPase expression protein 2, mitochondrial (AEP2), partial CSB-YP461176SVP
CSB-EP461176SVP
CSB-BP461176SVP
CSB-MP461176SVP
CSB-EP461176SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 3-ketoacyl-CoA reductase (SCRG_02811), partial CSB-YP461177SVP1
CSB-EP461177SVP1
CSB-BP461177SVP1
CSB-MP461177SVP1
CSB-EP461177SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 3 (AIM3), partial CSB-YP461178SVP
CSB-EP461178SVP
CSB-BP461178SVP
CSB-MP461178SVP
CSB-EP461178SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar membrane protein SCRG_03194 (SCRG_03194), partial CSB-YP461179SVP1
CSB-EP461179SVP1
CSB-BP461179SVP1
CSB-MP461179SVP1
CSB-EP461179SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GTP-binding protein YPT11 (YPT11) CSB-YP461180SVP
CSB-EP461180SVP
CSB-BP461180SVP
CSB-MP461180SVP
CSB-EP461180SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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