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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Beijerinckia indica subsp. indica 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP462162BSP
CSB-EP462162BSP
CSB-BP462162BSP
CSB-MP462162BSP
CSB-EP462162BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Ribosome maturation factor RimM (rimM) CSB-YP462163BSP
CSB-EP462163BSP
CSB-BP462163BSP
CSB-MP462163BSP
CSB-EP462163BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L27 (rpmA) CSB-YP462164BSP
CSB-EP462164BSP
CSB-BP462164BSP
CSB-MP462164BSP
CSB-EP462164BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP462165BSP
CSB-EP462165BSP
CSB-BP462165BSP
CSB-MP462165BSP
CSB-EP462165BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S11 (rpsK) CSB-YP462166BSP
CSB-EP462166BSP
CSB-BP462166BSP
CSB-MP462166BSP
CSB-EP462166BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L19 (rplS) CSB-YP462167BSP
CSB-EP462167BSP
CSB-BP462167BSP
CSB-MP462167BSP
CSB-EP462167BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica DNA ligase (ligA), partial CSB-YP462168BSP
CSB-EP462168BSP
CSB-BP462168BSP
CSB-MP462168BSP
CSB-EP462168BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP462169BSP
CSB-EP462169BSP
CSB-BP462169BSP
CSB-MP462169BSP
CSB-EP462169BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Probable phosphoketolase (Bind_2204), partial CSB-YP462170BSP
CSB-EP462170BSP
CSB-BP462170BSP
CSB-MP462170BSP
CSB-EP462170BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP462171BSP
CSB-EP462171BSP
CSB-BP462171BSP
CSB-MP462171BSP
CSB-EP462171BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase (Bind_0814) CSB-YP462172BSP
CSB-EP462172BSP
CSB-BP462172BSP
CSB-MP462172BSP
CSB-EP462172BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 50S ribosomal protein L9 (rplI) CSB-YP462173BSP
CSB-EP462173BSP
CSB-BP462173BSP
CSB-MP462173BSP
CSB-EP462173BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP462174BSP
CSB-EP462174BSP
CSB-BP462174BSP
CSB-MP462174BSP
CSB-EP462174BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP462175BSP
CSB-EP462175BSP
CSB-BP462175BSP
CSB-MP462175BSP
CSB-EP462175BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB, partial CSB-YP462176BSP
CSB-EP462176BSP
CSB-BP462176BSP
CSB-MP462176BSP
CSB-EP462176BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Probable acetoacetate decarboxylase (adc) CSB-YP462177BSP
CSB-EP462177BSP
CSB-BP462177BSP
CSB-MP462177BSP
CSB-EP462177BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S4 (rpsD) CSB-YP462178BSP
CSB-EP462178BSP
CSB-BP462178BSP
CSB-MP462178BSP
CSB-EP462178BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica ATP synthase subunit b 2 (atpF2), partial CSB-YP462179BSP
CSB-EP462179BSP
CSB-BP462179BSP
CSB-MP462179BSP
CSB-EP462179BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S12 (rpsL) CSB-YP462180BSP
CSB-EP462180BSP
CSB-BP462180BSP
CSB-MP462180BSP
CSB-EP462180BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S19 (rpsS) CSB-YP462181BSP
CSB-EP462181BSP
CSB-BP462181BSP
CSB-MP462181BSP
CSB-EP462181BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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