Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phytoplasma mali 30S ribosomal protein S12 (rpsL) CSB-YP463322EVH
CSB-EP463322EVH
CSB-BP463322EVH
CSB-MP463322EVH
CSB-EP463322EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali ATP-dependent zinc metalloprotease FtsH 2 (ftsH2), partial CSB-YP463323EVH
CSB-EP463323EVH
CSB-BP463323EVH
CSB-MP463323EVH
CSB-EP463323EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 30S ribosomal protein S4 (rpsD) CSB-YP463324EVH
CSB-EP463324EVH
CSB-BP463324EVH
CSB-MP463324EVH
CSB-EP463324EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L22 (rplV) CSB-YP463325EVH
CSB-EP463325EVH
CSB-BP463325EVH
CSB-MP463325EVH
CSB-EP463325EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L15 (rplO) CSB-YP463326EVH
CSB-EP463326EVH
CSB-BP463326EVH
CSB-MP463326EVH
CSB-EP463326EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L17 (rplQ) CSB-YP463327EVH
CSB-EP463327EVH
CSB-BP463327EVH
CSB-MP463327EVH
CSB-EP463327EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Cytidylate kinase (cmk) CSB-YP463328EVH
CSB-EP463328EVH
CSB-BP463328EVH
CSB-MP463328EVH
CSB-EP463328EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali tRNA (guanine-N (1)-)-methyltransferase CSB-YP463329EVH
CSB-EP463329EVH
CSB-BP463329EVH
CSB-MP463329EVH
CSB-EP463329EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Ribosome maturation factor RimP (rimP) CSB-YP463330DZS
CSB-EP463330DZS
CSB-BP463330DZS
CSB-MP463330DZS
CSB-EP463330DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Phosphoglucosamine mutase (glmM) CSB-YP463331DZS
CSB-EP463331DZS
CSB-BP463331DZS
CSB-MP463331DZS
CSB-EP463331DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP463332DZS
CSB-EP463332DZS
CSB-BP463332DZS
CSB-MP463332DZS
CSB-EP463332DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP463333DZS
CSB-EP463333DZS
CSB-BP463333DZS
CSB-MP463333DZS
CSB-EP463333DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 30S ribosomal protein S18 (rpsR) CSB-YP463334DZS
CSB-EP463334DZS
CSB-BP463334DZS
CSB-MP463334DZS
CSB-EP463334DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Ribosome maturation factor RimM (rimM) CSB-YP463335DZS
CSB-EP463335DZS
CSB-BP463335DZS
CSB-MP463335DZS
CSB-EP463335DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP463336DZS
CSB-EP463336DZS
CSB-BP463336DZS
CSB-MP463336DZS
CSB-EP463336DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP463337DZS
CSB-EP463337DZS
CSB-BP463337DZS
CSB-MP463337DZS
CSB-EP463337DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Protein grpE (grpE) CSB-YP463338DZS
CSB-EP463338DZS
CSB-BP463338DZS
CSB-MP463338DZS
CSB-EP463338DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP463339DZS
CSB-EP463339DZS
CSB-BP463339DZS
CSB-MP463339DZS
CSB-EP463339DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP463340DZS
CSB-EP463340DZS
CSB-BP463340DZS
CSB-MP463340DZS
CSB-EP463340DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Probable intracellular septation protein A (RALTA_A1457), partial CSB-YP463341DZS1
CSB-EP463341DZS1
CSB-BP463341DZS1
CSB-MP463341DZS1
CSB-EP463341DZS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>