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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nosema bombycis Spore wall protein 12 (SWP12) CSB-YP463362NHN
CSB-EP463362NHN
CSB-BP463362NHN
CSB-MP463362NHN
CSB-EP463362NHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP463363BVS1
CSB-EP463363BVS1
CSB-BP463363BVS1
CSB-MP463363BVS1
CSB-EP463363BVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon NAD (P)H-quinone oxidoreductase subunit 5, chloroplastic, partial CSB-YP463364BVS
CSB-EP463364BVS
CSB-BP463364BVS
CSB-MP463364BVS
CSB-EP463364BVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brachypodium distachyon Photosystem II D2 protein (psbD), partial CSB-YP463365BVS1
CSB-EP463365BVS1
CSB-BP463365BVS1
CSB-MP463365BVS1
CSB-EP463365BVS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Chromosomal replication initiator protein DnaA (dnaA) CSB-YP463366LMJ
CSB-EP463366LMJ
CSB-BP463366LMJ
CSB-MP463366LMJ
CSB-EP463366LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Probable manganese transport protein MntH (mntH), partial CSB-YP463367LMJ
CSB-EP463367LMJ
CSB-BP463367LMJ
CSB-MP463367LMJ
CSB-EP463367LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Hydroxyethylthiazole kinase (thiM) CSB-YP463368LMJ
CSB-EP463368LMJ
CSB-BP463368LMJ
CSB-MP463368LMJ
CSB-EP463368LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Inosose dehydratase (iolE) CSB-YP463369LMJ
CSB-EP463369LMJ
CSB-BP463369LMJ
CSB-MP463369LMJ
CSB-EP463369LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP463370LMJ
CSB-EP463370LMJ
CSB-BP463370LMJ
CSB-MP463370LMJ
CSB-EP463370LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 60 kDa chaperonin (groL), partial CSB-BP463371LMJ
CSB-MP463371LMJ
CSB-EP463371LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Glutamate--tRNA ligase (gltX) CSB-YP463372LMJ
CSB-EP463372LMJ
CSB-BP463372LMJ
CSB-MP463372LMJ
CSB-EP463372LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP463373LMJ
CSB-EP463373LMJ
CSB-BP463373LMJ
CSB-MP463373LMJ
CSB-EP463373LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L36 (rpmJ) CSB-YP463374LMJ
CSB-EP463374LMJ
CSB-BP463374LMJ
CSB-MP463374LMJ
CSB-EP463374LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L5 (rplE) CSB-YP463375LMJ
CSB-EP463375LMJ
CSB-BP463375LMJ
CSB-MP463375LMJ
CSB-EP463375LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S19 (rpsS) CSB-YP463376LMJ
CSB-EP463376LMJ
CSB-BP463376LMJ
CSB-MP463376LMJ
CSB-EP463376LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP463377LMJ
CSB-EP463377LMJ
CSB-BP463377LMJ
CSB-MP463377LMJ
CSB-EP463377LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP463378LMJ
CSB-EP463378LMJ
CSB-BP463378LMJ
CSB-MP463378LMJ
CSB-EP463378LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Phosphoglucosamine mutase (glmM) CSB-YP463379LMJ
CSB-EP463379LMJ
CSB-BP463379LMJ
CSB-MP463379LMJ
CSB-EP463379LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Xanthine phosphoribosyltransferase (xpt) CSB-YP463380LMJ
CSB-EP463380LMJ
CSB-BP463380LMJ
CSB-MP463380LMJ
CSB-EP463380LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP463381LMJ
CSB-EP463381LMJ
CSB-BP463381LMJ
CSB-MP463381LMJ
CSB-EP463381LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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