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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S8P (rps8p) CSB-YP463722TNE
CSB-EP463722TNE
CSB-BP463722TNE
CSB-MP463722TNE
CSB-EP463722TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus UPF0219 protein Tneu_1356 (Tneu_1356) CSB-YP463723TNE
CSB-EP463723TNE
CSB-BP463723TNE
CSB-MP463723TNE
CSB-EP463723TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus DNA-directed RNA polymerase subunit P (rpoP) CSB-YP463724TNE
CSB-EP463724TNE
CSB-BP463724TNE
CSB-MP463724TNE
CSB-EP463724TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP463725TNE
CSB-EP463725TNE
CSB-BP463725TNE
CSB-MP463725TNE
CSB-EP463725TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S28e (rps28e) CSB-YP463726TNE
CSB-EP463726TNE
CSB-BP463726TNE
CSB-MP463726TNE
CSB-EP463726TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Transcription factor E (tfe) CSB-YP463727TNE
CSB-EP463727TNE
CSB-BP463727TNE
CSB-MP463727TNE
CSB-EP463727TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus DNA ligase (lig), partial CSB-YP463728TNE
CSB-EP463728TNE
CSB-BP463728TNE
CSB-MP463728TNE
CSB-EP463728TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Geranylgeranylglyceryl phosphate synthase (Tneu_1677) CSB-YP463729TNE
CSB-EP463729TNE
CSB-BP463729TNE
CSB-MP463729TNE
CSB-EP463729TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Cytidylate kinase (cmk) CSB-YP463730TNE
CSB-EP463730TNE
CSB-BP463730TNE
CSB-MP463730TNE
CSB-EP463730TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S10P (rps10p) CSB-YP463731TNE
CSB-EP463731TNE
CSB-BP463731TNE
CSB-MP463731TNE
CSB-EP463731TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Probable non-canonical purine NTP phosphatase (Tneu_1859) CSB-YP463732TNE
CSB-EP463732TNE
CSB-BP463732TNE
CSB-MP463732TNE
CSB-EP463732TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 5-formaminoimidazole-4-carboxamide-1- (beta)-D-ribofuranosyl 5'-monophosphate synthetase CSB-YP463733TNE
CSB-EP463733TNE
CSB-BP463733TNE
CSB-MP463733TNE
CSB-EP463733TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L13P (rpl13p) CSB-YP463734TNE
CSB-EP463734TNE
CSB-BP463734TNE
CSB-MP463734TNE
CSB-EP463734TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Replication factor C large subunit (rfcL) CSB-YP463735TNE
CSB-EP463735TNE
CSB-BP463735TNE
CSB-MP463735TNE
CSB-EP463735TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP463736TNE
CSB-EP463736TNE
CSB-BP463736TNE
CSB-MP463736TNE
CSB-EP463736TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP463737TNE
CSB-EP463737TNE
CSB-BP463737TNE
CSB-MP463737TNE
CSB-EP463737TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus RNA-binding protein AU-1 (aubA) CSB-YP463738TNE
CSB-EP463738TNE
CSB-BP463738TNE
CSB-MP463738TNE
CSB-EP463738TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP463739EPT1
CSB-EP463739EPT1
CSB-BP463739EPT1
CSB-MP463739EPT1
CSB-EP463739EPT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Probable transaldolase (tal) CSB-YP463740EPT
CSB-EP463740EPT
CSB-BP463740EPT
CSB-MP463740EPT
CSB-EP463740EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum Arginine--tRNA ligase (argS), partial CSB-YP463741EPT
CSB-EP463741EPT
CSB-BP463741EPT
CSB-MP463741EPT
CSB-EP463741EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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