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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobaculum parvum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP463282DSQ
CSB-EP463282DSQ
CSB-BP463282DSQ
CSB-MP463282DSQ
CSB-EP463282DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Elongation factor 4 (lepA), partial CSB-YP463283DSQ
CSB-EP463283DSQ
CSB-BP463283DSQ
CSB-MP463283DSQ
CSB-EP463283DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum DNA mismatch repair protein MutS (mutS), partial CSB-YP463284DSQ
CSB-EP463284DSQ
CSB-BP463284DSQ
CSB-MP463284DSQ
CSB-EP463284DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP463285DSQ
CSB-EP463285DSQ
CSB-BP463285DSQ
CSB-MP463285DSQ
CSB-EP463285DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP463286DSQ
CSB-EP463286DSQ
CSB-BP463286DSQ
CSB-MP463286DSQ
CSB-EP463286DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Histidine--tRNA ligase (hisS) CSB-YP463287DSQ
CSB-EP463287DSQ
CSB-BP463287DSQ
CSB-MP463287DSQ
CSB-EP463287DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L11 (rplK) CSB-YP463288DSQ
CSB-EP463288DSQ
CSB-BP463288DSQ
CSB-MP463288DSQ
CSB-EP463288DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Probable GTP-binding protein EngB (engB) CSB-YP463289DSQ
CSB-EP463289DSQ
CSB-BP463289DSQ
CSB-MP463289DSQ
CSB-EP463289DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum ATP phosphoribosyltransferase (hisG) CSB-YP463290DSQ
CSB-EP463290DSQ
CSB-BP463290DSQ
CSB-MP463290DSQ
CSB-EP463290DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 30S ribosomal protein S10 (rpsJ) CSB-YP463291DSQ
CSB-EP463291DSQ
CSB-BP463291DSQ
CSB-MP463291DSQ
CSB-EP463291DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L18 (rplR) CSB-YP463292DSQ
CSB-EP463292DSQ
CSB-BP463292DSQ
CSB-MP463292DSQ
CSB-EP463292DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L15 (rplO) CSB-YP463293DSQ
CSB-EP463293DSQ
CSB-BP463293DSQ
CSB-MP463293DSQ
CSB-EP463293DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP463294DSQ
CSB-EP463294DSQ
CSB-BP463294DSQ
CSB-MP463294DSQ
CSB-EP463294DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum 50S ribosomal protein L20 (rplT) CSB-YP463295DSQ
CSB-EP463295DSQ
CSB-BP463295DSQ
CSB-MP463295DSQ
CSB-EP463295DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Probable transaldolase (tal) CSB-YP463296DSY
CSB-EP463296DSY
CSB-BP463296DSY
CSB-MP463296DSY
CSB-EP463296DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP463297DSY
CSB-EP463297DSY
CSB-BP463297DSY
CSB-MP463297DSY
CSB-EP463297DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Pantothenate synthetase (panC) CSB-YP463298DSY
CSB-EP463298DSY
CSB-BP463298DSY
CSB-MP463298DSY
CSB-EP463298DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 50S ribosomal protein L27 (rpmA) CSB-YP463299DSY
CSB-EP463299DSY
CSB-BP463299DSY
CSB-MP463299DSY
CSB-EP463299DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Methionine--tRNA ligase (metG), partial CSB-YP463300DSY
CSB-EP463300DSY
CSB-BP463300DSY
CSB-MP463300DSY
CSB-EP463300DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Protein grpE (grpE) CSB-YP463301DSY
CSB-EP463301DSY
CSB-BP463301DSY
CSB-MP463301DSY
CSB-EP463301DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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