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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Proteus mirabilis 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP463442EYZ
CSB-EP463442EYZ
CSB-BP463442EYZ
CSB-MP463442EYZ
CSB-EP463442EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Deoxyribose-phosphate aldolase (deoC) CSB-YP463443EYZ
CSB-EP463443EYZ
CSB-BP463443EYZ
CSB-MP463443EYZ
CSB-EP463443EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Anthranilate phosphoribosyltransferase (trpD) CSB-YP463444EYZ
CSB-EP463444EYZ
CSB-BP463444EYZ
CSB-MP463444EYZ
CSB-EP463444EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis HTH-type transcriptional repressor PurR (purR) CSB-YP463445EYZ
CSB-EP463445EYZ
CSB-BP463445EYZ
CSB-MP463445EYZ
CSB-EP463445EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Fatty acid metabolism regulator protein (fadR) CSB-YP463446EYZ
CSB-EP463446EYZ
CSB-BP463446EYZ
CSB-MP463446EYZ
CSB-EP463446EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Carnitinyl-CoA dehydratase (caiD) CSB-YP463447EYZ
CSB-EP463447EYZ
CSB-BP463447EYZ
CSB-MP463447EYZ
CSB-EP463447EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP463448EYZ
CSB-EP463448EYZ
CSB-BP463448EYZ
CSB-MP463448EYZ
CSB-EP463448EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis UPF0059 membrane protein PMI1608 (PMI1608), partial CSB-YP463449EYZ1
CSB-EP463449EYZ1
CSB-BP463449EYZ1
CSB-MP463449EYZ1
CSB-EP463449EYZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP463450EYZ
CSB-EP463450EYZ
CSB-BP463450EYZ
CSB-MP463450EYZ
CSB-EP463450EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 30S ribosomal protein S7 (rpsG) CSB-YP463451EYZ
CSB-EP463451EYZ
CSB-BP463451EYZ
CSB-MP463451EYZ
CSB-EP463451EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP463452EYZ
CSB-EP463452EYZ
CSB-BP463452EYZ
CSB-MP463452EYZ
CSB-EP463452EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP463453EYZ
CSB-EP463453EYZ
CSB-BP463453EYZ
CSB-MP463453EYZ
CSB-EP463453EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Glutamate--tRNA ligase (gltX) CSB-YP463454EYZ
CSB-EP463454EYZ
CSB-BP463454EYZ
CSB-MP463454EYZ
CSB-EP463454EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP463455EYZ
CSB-EP463455EYZ
CSB-BP463455EYZ
CSB-MP463455EYZ
CSB-EP463455EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 33 kDa chaperonin (hslO) CSB-YP463456EYZ
CSB-EP463456EYZ
CSB-BP463456EYZ
CSB-MP463456EYZ
CSB-EP463456EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis SsrA-binding protein (smpB) CSB-YP463457EYZ
CSB-EP463457EYZ
CSB-BP463457EYZ
CSB-MP463457EYZ
CSB-EP463457EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Lysine--tRNA ligase (lysS), partial CSB-YP463458EYZ
CSB-EP463458EYZ
CSB-BP463458EYZ
CSB-MP463458EYZ
CSB-EP463458EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Protein FdhE homolog (fdhE) CSB-YP463459EYZ
CSB-EP463459EYZ
CSB-BP463459EYZ
CSB-MP463459EYZ
CSB-EP463459EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis DNA replication and repair protein recF (recF) CSB-YP463460EYZ
CSB-EP463460EYZ
CSB-BP463460EYZ
CSB-MP463460EYZ
CSB-EP463460EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis 50S ribosomal protein L33 (rpmG) CSB-YP463461EYZ
CSB-EP463461EYZ
CSB-BP463461EYZ
CSB-MP463461EYZ
CSB-EP463461EYZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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