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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia pickettii Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP464642RJI
CSB-EP464642RJI
CSB-BP464642RJI
CSB-MP464642RJI
CSB-EP464642RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP464643RJI
CSB-EP464643RJI
CSB-BP464643RJI
CSB-MP464643RJI
CSB-EP464643RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP464644RJI
CSB-EP464644RJI
CSB-BP464644RJI
CSB-MP464644RJI
CSB-EP464644RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Indole-3-glycerol phosphate synthase (trpC) CSB-YP464645RJI
CSB-EP464645RJI
CSB-BP464645RJI
CSB-MP464645RJI
CSB-EP464645RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP464646RJI
CSB-EP464646RJI
CSB-BP464646RJI
CSB-MP464646RJI
CSB-EP464646RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP464647RJI
CSB-EP464647RJI
CSB-BP464647RJI
CSB-MP464647RJI
CSB-EP464647RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L6 (rplF) CSB-YP464648RJI
CSB-EP464648RJI
CSB-BP464648RJI
CSB-MP464648RJI
CSB-EP464648RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L5 (rplE) CSB-YP464649RJI
CSB-EP464649RJI
CSB-BP464649RJI
CSB-MP464649RJI
CSB-EP464649RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L2 (rplB) CSB-YP464650RJI
CSB-EP464650RJI
CSB-BP464650RJI
CSB-MP464650RJI
CSB-EP464650RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L3 (rplC) CSB-YP464651RJI
CSB-EP464651RJI
CSB-BP464651RJI
CSB-MP464651RJI
CSB-EP464651RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L7/L12 (rplL) CSB-YP464652RJI
CSB-EP464652RJI
CSB-BP464652RJI
CSB-MP464652RJI
CSB-EP464652RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP464653RJI
CSB-EP464653RJI
CSB-BP464653RJI
CSB-MP464653RJI
CSB-EP464653RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L11 (rplK) CSB-YP464654RJI
CSB-EP464654RJI
CSB-BP464654RJI
CSB-MP464654RJI
CSB-EP464654RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP464655RJI
CSB-EP464655RJI
CSB-BP464655RJI
CSB-MP464655RJI
CSB-EP464655RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Putative arginyl-tRNA--protein transferase (ate) CSB-YP464656RJI
CSB-EP464656RJI
CSB-BP464656RJI
CSB-MP464656RJI
CSB-EP464656RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L35 (rpmI) CSB-YP464657RJI
CSB-EP464657RJI
CSB-BP464657RJI
CSB-MP464657RJI
CSB-EP464657RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 1-aminocyclopropane-1-carboxylate deaminase (acdS) CSB-YP464658RJI
CSB-EP464658RJI
CSB-BP464658RJI
CSB-MP464658RJI
CSB-EP464658RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP464659RJI
CSB-EP464659RJI
CSB-BP464659RJI
CSB-MP464659RJI
CSB-EP464659RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Flagellar transcriptional regulator FlhD (flhD) CSB-YP464660RJI
CSB-EP464660RJI
CSB-BP464660RJI
CSB-MP464660RJI
CSB-EP464660RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Homogentisate 1,2-dioxygenase (hmgA) CSB-YP464661RJI
CSB-EP464661RJI
CSB-BP464661RJI
CSB-MP464661RJI
CSB-EP464661RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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