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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Wolbachia pipientis subsp. Culex pipiens Histidine--tRNA ligase (hisS) CSB-YP464842WBO
CSB-EP464842WBO
CSB-BP464842WBO
CSB-MP464842WBO
CSB-EP464842WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens ATP-dependent protease subunit HslV (hslV) CSB-YP464843WBO
CSB-EP464843WBO
CSB-BP464843WBO
CSB-MP464843WBO
CSB-EP464843WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP464844WBO
CSB-EP464844WBO
CSB-BP464844WBO
CSB-MP464844WBO
CSB-EP464844WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens tRNA (guanine-N (1)-)-methyltransferase CSB-YP464845WBO
CSB-EP464845WBO
CSB-BP464845WBO
CSB-MP464845WBO
CSB-EP464845WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Protein RecA (recA) CSB-YP464846WBO
CSB-EP464846WBO
CSB-BP464846WBO
CSB-MP464846WBO
CSB-EP464846WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Threonine--tRNA ligase (thrS), partial CSB-YP464847WBO
CSB-EP464847WBO
CSB-BP464847WBO
CSB-MP464847WBO
CSB-EP464847WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP464848WBO
CSB-EP464848WBO
CSB-BP464848WBO
CSB-MP464848WBO
CSB-EP464848WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L16 (rplP) CSB-YP464849WBO
CSB-EP464849WBO
CSB-BP464849WBO
CSB-MP464849WBO
CSB-EP464849WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 30S ribosomal protein S14 (rpsN) CSB-YP464850WBO
CSB-EP464850WBO
CSB-BP464850WBO
CSB-MP464850WBO
CSB-EP464850WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L17 (rplQ) CSB-YP464851WBO
CSB-EP464851WBO
CSB-BP464851WBO
CSB-MP464851WBO
CSB-EP464851WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP464852WBO
CSB-EP464852WBO
CSB-BP464852WBO
CSB-MP464852WBO
CSB-EP464852WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Arginine--tRNA ligase (argS), partial CSB-YP464853WBO
CSB-EP464853WBO
CSB-BP464853WBO
CSB-MP464853WBO
CSB-EP464853WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens DNA repair protein recO (recO) CSB-YP464854WBO
CSB-EP464854WBO
CSB-BP464854WBO
CSB-MP464854WBO
CSB-EP464854WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Elongation factor P (efp) CSB-YP464855OEV
CSB-EP464855OEV
CSB-BP464855OEV
CSB-MP464855OEV
CSB-EP464855OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP464856OEV
CSB-EP464856OEV
CSB-BP464856OEV
CSB-MP464856OEV
CSB-EP464856OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP464857OEV
CSB-EP464857OEV
CSB-BP464857OEV
CSB-MP464857OEV
CSB-EP464857OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi Cysteine--tRNA ligase (cysS) CSB-YP464858OEV
CSB-EP464858OEV
CSB-BP464858OEV
CSB-MP464858OEV
CSB-EP464858OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 50S ribosomal protein L9 (rplI) CSB-YP464859OEV
CSB-EP464859OEV
CSB-BP464859OEV
CSB-MP464859OEV
CSB-EP464859OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP464860OEV
CSB-EP464860OEV
CSB-BP464860OEV
CSB-MP464860OEV
CSB-EP464860OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi 30S ribosomal protein S12 (rpsL) CSB-YP464861OEV
CSB-EP464861OEV
CSB-BP464861OEV
CSB-MP464861OEV
CSB-EP464861OEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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