Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptothrix cholodnii 50S ribosomal protein L33 (rpmG) CSB-YP463702LOT
CSB-EP463702LOT
CSB-BP463702LOT
CSB-MP463702LOT
CSB-EP463702LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Glycerol kinase (glpK) CSB-YP463703LOT
CSB-EP463703LOT
CSB-BP463703LOT
CSB-MP463703LOT
CSB-EP463703LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii GTPase obg (obg) CSB-YP463704LOT
CSB-EP463704LOT
CSB-BP463704LOT
CSB-MP463704LOT
CSB-EP463704LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP463705LOT
CSB-EP463705LOT
CSB-BP463705LOT
CSB-MP463705LOT
CSB-EP463705LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Hydroxyethylthiazole kinase (thiM) CSB-YP463706LOT
CSB-EP463706LOT
CSB-BP463706LOT
CSB-MP463706LOT
CSB-EP463706LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L25 (rplY) CSB-YP463707LOT
CSB-EP463707LOT
CSB-BP463707LOT
CSB-MP463707LOT
CSB-EP463707LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Urease subunit beta (ureB) CSB-YP463708LOT
CSB-EP463708LOT
CSB-BP463708LOT
CSB-MP463708LOT
CSB-EP463708LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP463709LOT
CSB-EP463709LOT
CSB-BP463709LOT
CSB-MP463709LOT
CSB-EP463709LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Phosphomethylpyrimidine synthase (thiC), partial CSB-YP463710LOT
CSB-EP463710LOT
CSB-BP463710LOT
CSB-MP463710LOT
CSB-EP463710LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Periplasmic nitrate reductase (napA), partial CSB-YP463711LOT
CSB-EP463711LOT
CSB-BP463711LOT
CSB-MP463711LOT
CSB-EP463711LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP463712LOT
CSB-EP463712LOT
CSB-BP463712LOT
CSB-MP463712LOT
CSB-EP463712LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii ATP-dependent protease subunit HslV (hslV) CSB-YP463713LOT
CSB-EP463713LOT
CSB-BP463713LOT
CSB-MP463713LOT
CSB-EP463713LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Dihydrodipicolinate synthase (dapA) CSB-YP463714LOT
CSB-EP463714LOT
CSB-BP463714LOT
CSB-MP463714LOT
CSB-EP463714LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L11 (rplK) CSB-YP463715LOT
CSB-EP463715LOT
CSB-BP463715LOT
CSB-MP463715LOT
CSB-EP463715LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Catalase-peroxidase (katG), partial CSB-YP463716LOT
CSB-EP463716LOT
CSB-BP463716LOT
CSB-MP463716LOT
CSB-EP463716LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii UPF0246 protein Lcho_2652 (Lcho_2652) CSB-YP463717LOT
CSB-EP463717LOT
CSB-BP463717LOT
CSB-MP463717LOT
CSB-EP463717LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L36 (rpmJ) CSB-YP463718LOT
CSB-EP463718LOT
CSB-BP463718LOT
CSB-MP463718LOT
CSB-EP463718LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L14 (rplN) CSB-YP463719LOT
CSB-EP463719LOT
CSB-BP463719LOT
CSB-MP463719LOT
CSB-EP463719LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S3 (rpsC) CSB-YP463720LOT
CSB-EP463720LOT
CSB-BP463720LOT
CSB-MP463720LOT
CSB-EP463720LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 30S ribosomal protein S27ae (rps27Ae) CSB-YP463721TNE
CSB-EP463721TNE
CSB-BP463721TNE
CSB-MP463721TNE
CSB-EP463721TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>