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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia ambifaria Urease subunit alpha (ureC), partial CSB-YP463802BXP
CSB-EP463802BXP
CSB-BP463802BXP
CSB-MP463802BXP
CSB-EP463802BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Cell division topological specificity factor (minE) CSB-YP463803BXP
CSB-EP463803BXP
CSB-BP463803BXP
CSB-MP463803BXP
CSB-EP463803BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Cytidylate kinase (cmk) CSB-YP463804BXP
CSB-EP463804BXP
CSB-BP463804BXP
CSB-MP463804BXP
CSB-EP463804BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L19 (rplS) CSB-YP463805BXP
CSB-EP463805BXP
CSB-BP463805BXP
CSB-MP463805BXP
CSB-EP463805BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Lipoprotein signal peptidase (lspA), partial CSB-YP463806BXP1
CSB-EP463806BXP1
CSB-BP463806BXP1
CSB-MP463806BXP1
CSB-EP463806BXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP463807BXP
CSB-EP463807BXP
CSB-BP463807BXP
CSB-MP463807BXP
CSB-EP463807BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 50S ribosomal protein L32 (rpmF) CSB-YP463808BXP
CSB-EP463808BXP
CSB-BP463808BXP
CSB-MP463808BXP
CSB-EP463808BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP463809BXP
CSB-EP463809BXP
CSB-BP463809BXP
CSB-MP463809BXP
CSB-EP463809BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP463810BXP
CSB-EP463810BXP
CSB-BP463810BXP
CSB-MP463810BXP
CSB-EP463810BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria UPF0145 protein BamMC406_5002 (BamMC406_5002) CSB-YP463811BXP
CSB-EP463811BXP
CSB-BP463811BXP
CSB-MP463811BXP
CSB-EP463811BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP463812BXP
CSB-EP463812BXP
CSB-BP463812BXP
CSB-MP463812BXP
CSB-EP463812BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria 3-deoxy-manno-octulosonate cytidylyltransferase 2 (kdsB2) CSB-YP463813BXP
CSB-EP463813BXP
CSB-BP463813BXP
CSB-MP463813BXP
CSB-EP463813BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Adenylate kinase (adk) CSB-YP463814MTC
CSB-EP463814MTC
CSB-BP463814MTC
CSB-MP463814MTC
CSB-EP463814MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 50S ribosomal protein L24 (rplX) CSB-YP463815MTC
CSB-EP463815MTC
CSB-BP463815MTC
CSB-MP463815MTC
CSB-EP463815MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 50S ribosomal protein L32 (rpmF) CSB-YP463816MTC
CSB-EP463816MTC
CSB-BP463816MTC
CSB-MP463816MTC
CSB-EP463816MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi 50S ribosomal protein L34 (rpmH) CSB-YP463817MTC
CSB-EP463817MTC
CSB-BP463817MTC
CSB-MP463817MTC
CSB-EP463817MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP463818MTC
CSB-EP463818MTC
CSB-BP463818MTC
CSB-MP463818MTC
CSB-EP463818MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi DNA ligase (ligA), partial CSB-YP463819MTC
CSB-EP463819MTC
CSB-BP463819MTC
CSB-MP463819MTC
CSB-EP463819MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP463820MTC
CSB-EP463820MTC
CSB-BP463820MTC
CSB-MP463820MTC
CSB-EP463820MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP463821MTC
CSB-EP463821MTC
CSB-BP463821MTC
CSB-MP463821MTC
CSB-EP463821MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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