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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cupriavidus taiwanensis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP463882DZS
CSB-EP463882DZS
CSB-BP463882DZS
CSB-MP463882DZS
CSB-EP463882DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 4-hydroxy-2-oxovalerate aldolase (mhpE) CSB-YP463883DZS
CSB-EP463883DZS
CSB-BP463883DZS
CSB-MP463883DZS
CSB-EP463883DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Golgi apparatus membrane protein TVP23 (TVP23), partial CSB-YP463884EXJ1
CSB-EP463884EXJ1
CSB-BP463884EXJ1
CSB-MP463884EXJ1
CSB-EP463884EXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Actin cytoskeleton-regulatory complex protein END3 (END3) CSB-YP463885EXJ
CSB-EP463885EXJ
CSB-BP463885EXJ
CSB-MP463885EXJ
CSB-EP463885EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP463886EXJ
CSB-EP463886EXJ
CSB-BP463886EXJ
CSB-MP463886EXJ
CSB-EP463886EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina 40S ribosomal protein S0 (RPS0) CSB-YP463887EXJ
CSB-EP463887EXJ
CSB-BP463887EXJ
CSB-MP463887EXJ
CSB-EP463887EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Leucine aminopeptidase 1 (LAP1) CSB-YP463888EXJ
CSB-EP463888EXJ
CSB-BP463888EXJ
CSB-MP463888EXJ
CSB-EP463888EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Ribosome biogenesis protein YTM1 (YTM1) CSB-YP463889EXJ
CSB-EP463889EXJ
CSB-BP463889EXJ
CSB-MP463889EXJ
CSB-EP463889EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquareovirus G Outer capsid protein VP1 (S1), partial CSB-YP463890DNU
CSB-EP463890DNU
CSB-BP463890DNU
CSB-MP463890DNU
CSB-EP463890DNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquareovirus G Outer capsid protein VP4 (S6), partial CSB-YP463891DNU
CSB-EP463891DNU
CSB-BP463891DNU
CSB-MP463891DNU
CSB-EP463891DNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquareovirus G Non-structural 3 (S11) CSB-YP463892DNU
CSB-EP463892DNU
CSB-BP463892DNU
CSB-MP463892DNU
CSB-EP463892DNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chapare mammarenavirus Pre-glycoprotein polyprotein GP complex (GPC), partial CSB-YP463893DRQ1
CSB-BP463893DRQ1
CSB-EP463893DRQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phomopsis amygdali Geranylgeranyl pyrophosphate synthase D (GGS-D) CSB-YP463894EUU
CSB-EP463894EUU
CSB-BP463894EUU
CSB-MP463894EUU
CSB-EP463894EUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP463895FLX
CSB-EP463895FLX
CSB-BP463895FLX
CSB-MP463895FLX
CSB-EP463895FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Protein smg homolog (smg) CSB-YP463896FLX
CSB-EP463896FLX
CSB-BP463896FLX
CSB-MP463896FLX
CSB-EP463896FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Ribosomal protein L11 methyltransferase (prmA) CSB-YP463897FLX
CSB-EP463897FLX
CSB-BP463897FLX
CSB-MP463897FLX
CSB-EP463897FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 50S ribosomal protein L13 (rplM) CSB-YP463898FLX
CSB-EP463898FLX
CSB-BP463898FLX
CSB-MP463898FLX
CSB-EP463898FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 5'-nucleotidase surE (surE) CSB-YP463899FLX
CSB-EP463899FLX
CSB-BP463899FLX
CSB-MP463899FLX
CSB-EP463899FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Regulatory protein recX (recX) CSB-YP463900FLX
CSB-EP463900FLX
CSB-BP463900FLX
CSB-MP463900FLX
CSB-EP463900FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Pantothenate synthetase (panC) CSB-YP463901FLX
CSB-EP463901FLX
CSB-BP463901FLX
CSB-MP463901FLX
CSB-EP463901FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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