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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter pylori Aspartate carbamoyltransferase (pyrB) CSB-YP464682HUZ
CSB-EP464682HUZ
CSB-BP464682HUZ
CSB-MP464682HUZ
CSB-EP464682HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Lipid-A-disaccharide synthase (lpxB) CSB-YP464683HUZ
CSB-EP464683HUZ
CSB-BP464683HUZ
CSB-MP464683HUZ
CSB-EP464683HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori GMP reductase (guaC) CSB-YP464684HUZ
CSB-EP464684HUZ
CSB-BP464684HUZ
CSB-MP464684HUZ
CSB-EP464684HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP464685HUZ
CSB-EP464685HUZ
CSB-BP464685HUZ
CSB-MP464685HUZ
CSB-EP464685HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori D-alanine--D-alanine ligase (ddl) CSB-YP464686HUZ
CSB-EP464686HUZ
CSB-BP464686HUZ
CSB-MP464686HUZ
CSB-EP464686HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Adenylate kinase (adk) CSB-YP464687HUZ
CSB-EP464687HUZ
CSB-BP464687HUZ
CSB-MP464687HUZ
CSB-EP464687HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Dihydroorotase (pyrC) CSB-YP464688HUZ
CSB-EP464688HUZ
CSB-BP464688HUZ
CSB-MP464688HUZ
CSB-EP464688HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP464689HUZ
CSB-EP464689HUZ
CSB-BP464689HUZ
CSB-MP464689HUZ
CSB-EP464689HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Recombination protein RecR (recR) CSB-YP464690HUZ
CSB-EP464690HUZ
CSB-BP464690HUZ
CSB-MP464690HUZ
CSB-EP464690HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Protease HtpX homolog (htpX), partial CSB-YP464691HUZ1
CSB-EP464691HUZ1
CSB-BP464691HUZ1
CSB-MP464691HUZ1
CSB-EP464691HUZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP464692HUZ
CSB-EP464692HUZ
CSB-BP464692HUZ
CSB-MP464692HUZ
CSB-EP464692HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP464693HUZ
CSB-EP464693HUZ
CSB-BP464693HUZ
CSB-MP464693HUZ
CSB-EP464693HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori tRNA (guanine-N (1)-)-methyltransferase CSB-YP464694HUZ
CSB-EP464694HUZ
CSB-BP464694HUZ
CSB-MP464694HUZ
CSB-EP464694HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Histidine--tRNA ligase (hisS) CSB-YP464695HUZ
CSB-EP464695HUZ
CSB-BP464695HUZ
CSB-MP464695HUZ
CSB-EP464695HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L1 (rplA) CSB-YP464696HUZ
CSB-EP464696HUZ
CSB-BP464696HUZ
CSB-MP464696HUZ
CSB-EP464696HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Formamidase (amiF) CSB-YP464697HUZ
CSB-EP464697HUZ
CSB-BP464697HUZ
CSB-MP464697HUZ
CSB-EP464697HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP464698HUZ
CSB-EP464698HUZ
CSB-BP464698HUZ
CSB-MP464698HUZ
CSB-EP464698HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S4 (rpsD) CSB-YP464699HUZ
CSB-EP464699HUZ
CSB-BP464699HUZ
CSB-MP464699HUZ
CSB-EP464699HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S14 type Z (rpsZ) CSB-YP464700HUZ
CSB-EP464700HUZ
CSB-BP464700HUZ
CSB-MP464700HUZ
CSB-EP464700HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S3 (rpsC) CSB-YP464701HUZ
CSB-EP464701HUZ
CSB-BP464701HUZ
CSB-MP464701HUZ
CSB-EP464701HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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