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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nostoc punctiforme UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP464182NHQ
CSB-EP464182NHQ
CSB-BP464182NHQ
CSB-MP464182NHQ
CSB-EP464182NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L25 (rplY) CSB-YP464183NHQ
CSB-EP464183NHQ
CSB-BP464183NHQ
CSB-MP464183NHQ
CSB-EP464183NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Photosystem II reaction center protein T (psbT), partial CSB-YP464184NHQ
CSB-EP464184NHQ
CSB-BP464184NHQ
CSB-MP464184NHQ
CSB-EP464184NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Sugar fermentation stimulation protein homolog (sfsA) CSB-YP464185NHQ
CSB-EP464185NHQ
CSB-BP464185NHQ
CSB-MP464185NHQ
CSB-EP464185NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L11 (rplK) CSB-YP464186NHQ
CSB-EP464186NHQ
CSB-BP464186NHQ
CSB-MP464186NHQ
CSB-EP464186NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Protein CrcB homolog (crcB), partial CSB-YP464187BXT1
CSB-EP464187BXT1
CSB-BP464187BXT1
CSB-MP464187BXT1
CSB-EP464187BXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP464188BXT
CSB-EP464188BXT
CSB-BP464188BXT
CSB-MP464188BXT
CSB-EP464188BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Aspartate--tRNA ligase (aspS), partial CSB-YP464189BXT
CSB-EP464189BXT
CSB-BP464189BXT
CSB-MP464189BXT
CSB-EP464189BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP464190BXT
CSB-EP464190BXT
CSB-BP464190BXT
CSB-MP464190BXT
CSB-EP464190BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S15 (rpsO) CSB-YP464191BXT
CSB-EP464191BXT
CSB-BP464191BXT
CSB-MP464191BXT
CSB-EP464191BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP464192BXT
CSB-EP464192BXT
CSB-BP464192BXT
CSB-MP464192BXT
CSB-EP464192BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Uracil phosphoribosyltransferase (upp) CSB-YP464193BXT
CSB-EP464193BXT
CSB-BP464193BXT
CSB-MP464193BXT
CSB-EP464193BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum N utilization substance protein B homolog (nusB) CSB-YP464194BXT
CSB-EP464194BXT
CSB-BP464194BXT
CSB-MP464194BXT
CSB-EP464194BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Probable septum site-determining protein MinC (minC) CSB-YP464195BXT
CSB-EP464195BXT
CSB-BP464195BXT
CSB-MP464195BXT
CSB-EP464195BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP464196BXT
CSB-EP464196BXT
CSB-BP464196BXT
CSB-MP464196BXT
CSB-EP464196BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ribosome maturation factor RimM (rimM) CSB-YP464197BXT
CSB-EP464197BXT
CSB-BP464197BXT
CSB-MP464197BXT
CSB-EP464197BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0301 protein Bphy_2327 (Bphy_2327) CSB-YP464198BXT
CSB-EP464198BXT
CSB-BP464198BXT
CSB-MP464198BXT
CSB-EP464198BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP464199BXT
CSB-EP464199BXT
CSB-BP464199BXT
CSB-MP464199BXT
CSB-EP464199BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 30S ribosomal protein S6 (rpsF) CSB-YP464200BXT
CSB-EP464200BXT
CSB-BP464200BXT
CSB-MP464200BXT
CSB-EP464200BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP464201BXT
CSB-EP464201BXT
CSB-BP464201BXT
CSB-MP464201BXT
CSB-EP464201BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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