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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia phymatum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP464222BXT
CSB-EP464222BXT
CSB-BP464222BXT
CSB-MP464222BXT
CSB-EP464222BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Dihydroorotate dehydrogenase (quinone) CSB-YP464223BXT
CSB-EP464223BXT
CSB-BP464223BXT
CSB-MP464223BXT
CSB-EP464223BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum 4-hydroxy-2-oxovalerate aldolase 1 (Bphy_3510) CSB-YP464224BXT
CSB-EP464224BXT
CSB-BP464224BXT
CSB-MP464224BXT
CSB-EP464224BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum UPF0145 protein Bphy_3680 (Bphy_3680) CSB-YP464225BXT
CSB-EP464225BXT
CSB-BP464225BXT
CSB-MP464225BXT
CSB-EP464225BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum GTP cyclohydrolase folE2 (folE2) CSB-YP464226BXT
CSB-EP464226BXT
CSB-BP464226BXT
CSB-MP464226BXT
CSB-EP464226BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Fructose-1,6-bisphosphatase class 1 2 (fbp2) CSB-YP464227BXT
CSB-EP464227BXT
CSB-BP464227BXT
CSB-MP464227BXT
CSB-EP464227BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0758 protein YPTS_0048 (YPTS_0048) CSB-YP464228YAW
CSB-EP464228YAW
CSB-BP464228YAW
CSB-MP464228YAW
CSB-EP464228YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein-export protein secB (secB) CSB-YP464229YAW
CSB-EP464229YAW
CSB-BP464229YAW
CSB-MP464229YAW
CSB-EP464229YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0319 protein YPTS_1566 (YPTS_1566) CSB-YP464230YAW
CSB-EP464230YAW
CSB-BP464230YAW
CSB-MP464230YAW
CSB-EP464230YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP464231YAW
CSB-EP464231YAW
CSB-BP464231YAW
CSB-MP464231YAW
CSB-EP464231YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP464232YAW
CSB-EP464232YAW
CSB-BP464232YAW
CSB-MP464232YAW
CSB-EP464232YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Cell division protein ZapB (zapB) CSB-YP464233YAW
CSB-EP464233YAW
CSB-BP464233YAW
CSB-MP464233YAW
CSB-EP464233YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Glutamate racemase (murI) CSB-YP464234YAW
CSB-EP464234YAW
CSB-BP464234YAW
CSB-MP464234YAW
CSB-EP464234YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Uridine kinase (udk) CSB-YP464235YAW
CSB-EP464235YAW
CSB-BP464235YAW
CSB-MP464235YAW
CSB-EP464235YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ATP phosphoribosyltransferase (hisG) CSB-YP464236YAW
CSB-EP464236YAW
CSB-BP464236YAW
CSB-MP464236YAW
CSB-EP464236YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Putative ECA polymerase (wzyE), partial CSB-YP464237YAW
CSB-EP464237YAW
CSB-BP464237YAW
CSB-MP464237YAW
CSB-EP464237YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Sulfurtransferase TusA (tusA) CSB-YP464238YAW
CSB-EP464238YAW
CSB-BP464238YAW
CSB-MP464238YAW
CSB-EP464238YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0267 protein YPTS_1778 (YPTS_1778) CSB-YP464239YAW
CSB-EP464239YAW
CSB-BP464239YAW
CSB-MP464239YAW
CSB-EP464239YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Cell division protein ZapA (zapA) CSB-YP464240YAW
CSB-EP464240YAW
CSB-BP464240YAW
CSB-MP464240YAW
CSB-EP464240YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB UPF0301 protein YPTS_3341 (YPTS_3341) CSB-YP464241YAW
CSB-EP464241YAW
CSB-BP464241YAW
CSB-MP464241YAW
CSB-EP464241YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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