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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Beijerinckia indica subsp. indica Cysteine--tRNA ligase (cysS) CSB-YP464102BSP
CSB-EP464102BSP
CSB-BP464102BSP
CSB-MP464102BSP
CSB-EP464102BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Diaminopimelate epimerase (dapF) CSB-YP464103BSP
CSB-EP464103BSP
CSB-BP464103BSP
CSB-MP464103BSP
CSB-EP464103BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Aspartate 1-decarboxylase (panD) CSB-YP464104BSP
CSB-EP464104BSP
CSB-BP464104BSP
CSB-MP464104BSP
CSB-EP464104BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP464105BSP1
CSB-EP464105BSP1
CSB-BP464105BSP1
CSB-MP464105BSP1
CSB-EP464105BSP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica D-alanine--D-alanine ligase (ddl) CSB-YP464106BSP
CSB-EP464106BSP
CSB-BP464106BSP
CSB-MP464106BSP
CSB-EP464106BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP464107BSP
CSB-EP464107BSP
CSB-BP464107BSP
CSB-MP464107BSP
CSB-EP464107BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Trigger factor (tig) CSB-YP464108BSP
CSB-EP464108BSP
CSB-BP464108BSP
CSB-MP464108BSP
CSB-EP464108BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Ribonuclease PH (rph) CSB-YP464109BSP
CSB-EP464109BSP
CSB-BP464109BSP
CSB-MP464109BSP
CSB-EP464109BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP464110BSP1
CSB-EP464110BSP1
CSB-BP464110BSP1
CSB-MP464110BSP1
CSB-EP464110BSP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Ribosome maturation factor RimP (rimP) CSB-YP464111BSP
CSB-EP464111BSP
CSB-BP464111BSP
CSB-MP464111BSP
CSB-EP464111BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Arginine--tRNA ligase (argS), partial CSB-YP464112BSP
CSB-EP464112BSP
CSB-BP464112BSP
CSB-MP464112BSP
CSB-EP464112BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP464113BSP
CSB-EP464113BSP
CSB-BP464113BSP
CSB-MP464113BSP
CSB-EP464113BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S7 (rpsG) CSB-YP464114BSP
CSB-EP464114BSP
CSB-BP464114BSP
CSB-MP464114BSP
CSB-EP464114BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica 30S ribosomal protein S8 (rpsH) CSB-YP464115BSP
CSB-EP464115BSP
CSB-BP464115BSP
CSB-MP464115BSP
CSB-EP464115BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Adenylate kinase (adk) CSB-YP464116BSP
CSB-EP464116BSP
CSB-BP464116BSP
CSB-MP464116BSP
CSB-EP464116BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP464117BSP
CSB-EP464117BSP
CSB-BP464117BSP
CSB-MP464117BSP
CSB-EP464117BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Enolase (eno) CSB-YP464118BSP
CSB-EP464118BSP
CSB-BP464118BSP
CSB-MP464118BSP
CSB-EP464118BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP464119FMZ
CSB-EP464119FMZ
CSB-BP464119FMZ
CSB-MP464119FMZ
CSB-EP464119FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L28 (rpmB) CSB-YP464120FMZ
CSB-EP464120FMZ
CSB-BP464120FMZ
CSB-MP464120FMZ
CSB-EP464120FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Protein RecA (recA) CSB-YP464121FMZ
CSB-EP464121FMZ
CSB-BP464121FMZ
CSB-MP464121FMZ
CSB-EP464121FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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