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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Heterosigma akashiwo Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP464822HWH
CSB-EP464822HWH
CSB-BP464822HWH
CSB-MP464822HWH
CSB-EP464822HWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterosigma akashiwo ATP synthase subunit b, chloroplastic (atpF), partial CSB-YP464823HWG1
CSB-EP464823HWG1
CSB-BP464823HWG1
CSB-MP464823HWG1
CSB-EP464823HWG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Cytochrome c biogenesis protein ccsA (ccsA), partial CSB-YP464824FEC
CSB-EP464824FEC
CSB-BP464824FEC
CSB-MP464824FEC
CSB-EP464824FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale NAD (P)H-quinone oxidoreductase subunit J, chloroplastic CSB-YP464825FEC
CSB-EP464825FEC
CSB-BP464825FEC
CSB-MP464825FEC
CSB-EP464825FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale 50S ribosomal protein L20, chloroplastic (rpl20) CSB-YP464826FEC
CSB-EP464826FEC
CSB-BP464826FEC
CSB-MP464826FEC
CSB-EP464826FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Photosystem II reaction center protein I (psbI), partial CSB-YP464827FEC
CSB-EP464827FEC
CSB-BP464827FEC
CSB-MP464827FEC
CSB-EP464827FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fagopyrum esculentum subsp. ancestrale Protein psbN (psbN), partial CSB-YP464828FEC
CSB-EP464828FEC
CSB-BP464828FEC
CSB-MP464828FEC
CSB-EP464828FEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP464829WBL1
CSB-EP464829WBL1
CSB-BP464829WBL1
CSB-MP464829WBL1
CSB-EP464829WBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP464830WBL
CSB-EP464830WBL
CSB-BP464830WBL
CSB-MP464830WBL
CSB-EP464830WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Photosystem II reaction center protein K (psbK) CSB-YP464831WBL
CSB-EP464831WBL
CSB-BP464831WBL
CSB-MP464831WBL
CSB-EP464831WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Welwitschia mirabilis Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP464832WBL
CSB-EP464832WBL
CSB-BP464832WBL
CSB-MP464832WBL
CSB-EP464832WBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila teissieri Drosulfakinins (Dsk) CSB-YP464833DMO
CSB-EP464833DMO
CSB-BP464833DMO
CSB-MP464833DMO
CSB-EP464833DMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tabanus yao Apyrase, partial CSB-YP464834TKT
CSB-EP464834TKT
CSB-BP464834TKT
CSB-MP464834TKT
CSB-EP464834TKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Uncharacterized shell protein 4 CSB-YP464835LQP
CSB-EP464835LQP
CSB-BP464835LQP
CSB-MP464835LQP
CSB-EP464835LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Peroxidase-like protein 2 CSB-YP464836LQP
CSB-EP464836LQP
CSB-BP464836LQP
CSB-MP464836LQP
CSB-EP464836LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lottia gigantea Uncharacterized shell protein 22 CSB-YP464837LQP
CSB-EP464837LQP
CSB-BP464837LQP
CSB-MP464837LQP
CSB-EP464837LQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Maf-like protein WP0036 (WP0036) CSB-YP464838WBO
CSB-EP464838WBO
CSB-BP464838WBO
CSB-MP464838WBO
CSB-EP464838WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Trigger factor (tig) CSB-YP464839WBO
CSB-EP464839WBO
CSB-BP464839WBO
CSB-MP464839WBO
CSB-EP464839WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP464840WBO
CSB-EP464840WBO
CSB-BP464840WBO
CSB-MP464840WBO
CSB-EP464840WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens 50S ribosomal protein L35 (rpmI) CSB-YP464841WBO
CSB-EP464841WBO
CSB-BP464841WBO
CSB-MP464841WBO
CSB-EP464841WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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