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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae serotype 19F 30S ribosomal protein S16 (rpsP) CSB-YP466083FNE
CSB-EP466083FNE
CSB-BP466083FNE
CSB-MP466083FNE
CSB-EP466083FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Histidine--tRNA ligase (hisS), partial CSB-YP466084FNE
CSB-EP466084FNE
CSB-BP466084FNE
CSB-MP466084FNE
CSB-EP466084FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Arginine deiminase (arcA) CSB-YP466085FNE
CSB-EP466085FNE
CSB-BP466085FNE
CSB-MP466085FNE
CSB-EP466085FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP466086FNE
CSB-EP466086FNE
CSB-BP466086FNE
CSB-MP466086FNE
CSB-EP466086FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Glycerol kinase (glpK), partial CSB-YP466087FNE
CSB-EP466087FNE
CSB-BP466087FNE
CSB-MP466087FNE
CSB-EP466087FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 50S ribosomal protein L20 (rplT) CSB-YP466088FNE
CSB-EP466088FNE
CSB-BP466088FNE
CSB-MP466088FNE
CSB-EP466088FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Elongation factor 4 (lepA), partial CSB-YP466089FNE
CSB-EP466089FNE
CSB-BP466089FNE
CSB-MP466089FNE
CSB-EP466089FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 50S ribosomal protein L31 type B (rpmE2) CSB-YP466090FNE
CSB-EP466090FNE
CSB-BP466090FNE
CSB-MP466090FNE
CSB-EP466090FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Homoserine kinase (thrB) CSB-YP466091FNE
CSB-EP466091FNE
CSB-BP466091FNE
CSB-MP466091FNE
CSB-EP466091FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP466092FNE
CSB-EP466092FNE
CSB-BP466092FNE
CSB-MP466092FNE
CSB-EP466092FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 3-dehydroquinate synthase (aroB) CSB-YP466093FNE
CSB-EP466093FNE
CSB-BP466093FNE
CSB-MP466093FNE
CSB-EP466093FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F UPF0223 protein SPG_1345 (SPG_1345) CSB-YP466094FNE
CSB-EP466094FNE
CSB-BP466094FNE
CSB-MP466094FNE
CSB-EP466094FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F UPF0291 protein SPG_1401 (SPG_1401) CSB-YP466095FNE
CSB-EP466095FNE
CSB-BP466095FNE
CSB-MP466095FNE
CSB-EP466095FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 30S ribosomal protein S18 (rpsR) CSB-YP466096FNE
CSB-EP466096FNE
CSB-BP466096FNE
CSB-MP466096FNE
CSB-EP466096FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Putative Holliday junction resolvase (SPG_0181) CSB-YP466097FNE
CSB-EP466097FNE
CSB-BP466097FNE
CSB-MP466097FNE
CSB-EP466097FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 50S ribosomal protein L23 (rplW) CSB-YP466098FNE
CSB-EP466098FNE
CSB-BP466098FNE
CSB-MP466098FNE
CSB-EP466098FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 50S ribosomal protein L29 (rpmC) CSB-YP466099FNE
CSB-EP466099FNE
CSB-BP466099FNE
CSB-MP466099FNE
CSB-EP466099FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 50S ribosomal protein L18 (rplR) CSB-YP466100FNE
CSB-EP466100FNE
CSB-BP466100FNE
CSB-MP466100FNE
CSB-EP466100FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F UPF0210 protein SPG_0223 (SPG_0223) CSB-YP466101FNE
CSB-EP466101FNE
CSB-BP466101FNE
CSB-MP466101FNE
CSB-EP466101FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP466102FNE
CSB-EP466102FNE
CSB-BP466102FNE
CSB-MP466102FNE
CSB-EP466102FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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