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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP467083ENW
CSB-EP467083ENW
CSB-BP467083ENW
CSB-MP467083ENW
CSB-EP467083ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transport protein YidE (yidE), partial CSB-YP467084ENW
CSB-EP467084ENW
CSB-BP467084ENW
CSB-MP467084ENW
CSB-EP467084ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease P protein component (rnpA) CSB-YP467085ENW
CSB-EP467085ENW
CSB-BP467085ENW
CSB-MP467085ENW
CSB-EP467085ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bifunctional protein GlmU (glmU) CSB-YP467086ENW
CSB-EP467086ENW
CSB-BP467086ENW
CSB-MP467086ENW
CSB-EP467086ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0441 protein ygiB (ygiB) CSB-YP467087ENW
CSB-EP467087ENW
CSB-BP467087ENW
CSB-MP467087ENW
CSB-EP467087ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycogen synthesis protein glgS (glgS) CSB-YP467088ENW
CSB-EP467088ENW
CSB-BP467088ENW
CSB-MP467088ENW
CSB-EP467088ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP467089ENW1
CSB-EP467089ENW1
CSB-BP467089ENW1
CSB-MP467089ENW1
CSB-EP467089ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Threonine/serine transporter TdcC (tdcC), partial CSB-YP467090ENW
CSB-EP467090ENW
CSB-BP467090ENW
CSB-MP467090ENW
CSB-EP467090ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent RNA helicase rhlB (rhlB) CSB-YP467091ENW
CSB-EP467091ENW
CSB-BP467091ENW
CSB-MP467091ENW
CSB-EP467091ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) CSB-YP467092ENW
CSB-EP467092ENW
CSB-BP467092ENW
CSB-MP467092ENW
CSB-EP467092ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Der GTPase-activating protein YihI (yihI) CSB-YP467093ENW
CSB-EP467093ENW
CSB-BP467093ENW
CSB-MP467093ENW
CSB-EP467093ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional activator rhaS (rhaS) CSB-YP467094ENW
CSB-EP467094ENW
CSB-BP467094ENW
CSB-MP467094ENW
CSB-EP467094ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP-dependent protease subunit HslV (hslV) CSB-YP467095ENW
CSB-EP467095ENW
CSB-BP467095ENW
CSB-MP467095ENW
CSB-EP467095ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chorismate synthase (aroC) CSB-YP467096ENW
CSB-EP467096ENW
CSB-BP467096ENW
CSB-MP467096ENW
CSB-EP467096ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP467097ENW
CSB-EP467097ENW
CSB-BP467097ENW
CSB-MP467097ENW
CSB-EP467097ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP467098ENW
CSB-EP467098ENW
CSB-BP467098ENW
CSB-MP467098ENW
CSB-EP467098ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Iron-binding protein IscA (iscA) CSB-YP467099ENW
CSB-EP467099ENW
CSB-BP467099ENW
CSB-MP467099ENW
CSB-EP467099ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Elongation factor 4 (lepA) CSB-YP467100ENW
CSB-EP467100ENW
CSB-BP467100ENW
CSB-MP467100ENW
CSB-EP467100ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA (uracil (54)-C (5))-methyltransferase CSB-YP467101ENW
CSB-EP467101ENW
CSB-BP467101ENW
CSB-MP467101ENW
CSB-EP467101ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L7/L12 (rplL) CSB-YP467102ENW
CSB-EP467102ENW
CSB-BP467102ENW
CSB-MP467102ENW
CSB-EP467102ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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