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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Klebsiella pneumoniae UPF0250 protein KPK_3910 (KPK_3910) CSB-YP466683KBH
CSB-EP466683KBH
CSB-BP466683KBH
CSB-MP466683KBH
CSB-EP466683KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP466684KBH
CSB-EP466684KBH
CSB-BP466684KBH
CSB-MP466684KBH
CSB-EP466684KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Carboxylate-amine ligase KPK_4022 (KPK_4022) CSB-YP466685KBH
CSB-EP466685KBH
CSB-BP466685KBH
CSB-MP466685KBH
CSB-EP466685KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L31 type B (rpmE2) CSB-YP466686KBH
CSB-EP466686KBH
CSB-BP466686KBH
CSB-MP466686KBH
CSB-EP466686KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA sulfurtransferase (thiI) CSB-YP466687KBH
CSB-EP466687KBH
CSB-BP466687KBH
CSB-MP466687KBH
CSB-EP466687KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Acyl carrier protein phosphodiesterase (acpH) CSB-YP466688KBH
CSB-EP466688KBH
CSB-BP466688KBH
CSB-MP466688KBH
CSB-EP466688KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Anti-adapter protein iraP (iraP) CSB-YP466689KBH
CSB-EP466689KBH
CSB-BP466689KBH
CSB-MP466689KBH
CSB-EP466689KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein CsiD (csiD) CSB-YP466690KBH
CSB-EP466690KBH
CSB-BP466690KBH
CSB-MP466690KBH
CSB-EP466690KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ribonuclease HII (rnhB) CSB-YP466691KBH
CSB-EP466691KBH
CSB-BP466691KBH
CSB-MP466691KBH
CSB-EP466691KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP466692KBH
CSB-EP466692KBH
CSB-BP466692KBH
CSB-MP466692KBH
CSB-EP466692KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Glutamyl-Q tRNA (Asp) synthetase CSB-YP466693KBH
CSB-EP466693KBH
CSB-BP466693KBH
CSB-MP466693KBH
CSB-EP466693KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae DNA gyrase inhibitor YacG (yacG) CSB-YP466694KBH
CSB-EP466694KBH
CSB-BP466694KBH
CSB-MP466694KBH
CSB-EP466694KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP466695KBH
CSB-EP466695KBH
CSB-BP466695KBH
CSB-MP466695KBH
CSB-EP466695KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein ApaG (apaG) CSB-YP466696KBH
CSB-EP466696KBH
CSB-BP466696KBH
CSB-MP466696KBH
CSB-EP466696KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Dihydrodipicolinate reductase (dapB) CSB-YP466697KBH
CSB-EP466697KBH
CSB-BP466697KBH
CSB-MP466697KBH
CSB-EP466697KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Chaperone protein DnaJ (dnaJ) CSB-YP466698KBH
CSB-EP466698KBH
CSB-BP466698KBH
CSB-MP466698KBH
CSB-EP466698KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Thr operon leader peptide (thrL) CSB-YP466699KBH
CSB-EP466699KBH
CSB-BP466699KBH
CSB-MP466699KBH
CSB-EP466699KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Primosomal protein 1 (dnaT) CSB-YP466700KBH
CSB-EP466700KBH
CSB-BP466700KBH
CSB-MP466700KBH
CSB-EP466700KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP466701KBH
CSB-EP466701KBH
CSB-BP466701KBH
CSB-MP466701KBH
CSB-EP466701KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 60 kDa chaperonin (groL), partial CSB-YP466702KBH
CSB-EP466702KBH
CSB-BP466702KBH
CSB-MP466702KBH
CSB-EP466702KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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