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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces japonicus Elongation factor G, mitochondrial (mef1), partial CSB-YP467283SXT
CSB-EP467283SXT
CSB-BP467283SXT
CSB-MP467283SXT
CSB-EP467283SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dense granule protein 3 (GRA3), partial CSB-YP467284TOV1
CSB-BP467284TOV1
CSB-MP467284TOV1
CSB-EP467284TOV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei ATPase synthesis protein 25, mitochondrial (atp25), partial CSB-YP467285ETM
CSB-EP467285ETM
CSB-BP467285ETM
CSB-MP467285ETM
CSB-EP467285ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable E3 ubiquitin ligase complex SCF subunit sconB (sconB), partial CSB-YP467286ETM
CSB-EP467286ETM
CSB-BP467286ETM
CSB-MP467286ETM
CSB-EP467286ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Putative metallocarboxypeptidase ecm14 (ecm14), partial CSB-YP467287ETM
CSB-EP467287ETM
CSB-BP467287ETM
CSB-MP467287ETM
CSB-EP467287ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Nuclear distribution protein nudF 1 (nudF-1) CSB-YP467288ETM
CSB-EP467288ETM
CSB-BP467288ETM
CSB-MP467288ETM
CSB-EP467288ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (PMAA_083870), partial CSB-YP467289ETM
CSB-EP467289ETM
CSB-BP467289ETM
CSB-MP467289ETM
CSB-EP467289ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Probable carboxypeptidase PMAA_093910 (PMAA_093910) CSB-YP467290ETM
CSB-EP467290ETM
CSB-BP467290ETM
CSB-MP467290ETM
CSB-EP467290ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Mitochondrial distribution and morphology protein 34 (mdm34), partial CSB-YP467291ETM
CSB-EP467291ETM
CSB-BP467291ETM
CSB-MP467291ETM
CSB-EP467291ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Mannitol-1-phosphate 5-dehydrogenase (PMAA_048060) CSB-YP467292ETM
CSB-EP467292ETM
CSB-BP467292ETM
CSB-MP467292ETM
CSB-EP467292ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Pentafunctional AROM polypeptide 1 (aroM-1), partial CSB-YP467293ETM
CSB-EP467293ETM
CSB-BP467293ETM
CSB-MP467293ETM
CSB-EP467293ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Cell number regulator 6 (CNR6), partial CSB-YP467294ZAX1
CSB-EP467294ZAX1
CSB-BP467294ZAX1
CSB-MP467294ZAX1
CSB-EP467294ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CASP-like protein 7, partial CSB-YP467295ZAX1
CSB-EP467295ZAX1
CSB-BP467295ZAX1
CSB-MP467295ZAX1
CSB-EP467295ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Casparian strip membrane protein 1, partial CSB-YP467296ZAX1
CSB-EP467296ZAX1
CSB-BP467296ZAX1
CSB-MP467296ZAX1
CSB-EP467296ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Adenylyltransferase and sulfurtransferase MOCS3 1 (MOCS3-1) CSB-YP467297ZAX
CSB-EP467297ZAX
CSB-BP467297ZAX
CSB-MP467297ZAX
CSB-EP467297ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CASP-like protein 2, partial CSB-YP467298ZAX1
CSB-EP467298ZAX1
CSB-BP467298ZAX1
CSB-MP467298ZAX1
CSB-EP467298ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CASP-like protein 1, partial CSB-YP467299ZAX1
CSB-EP467299ZAX1
CSB-BP467299ZAX1
CSB-MP467299ZAX1
CSB-EP467299ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Ninja-family protein 4 CSB-YP467300ZAX
CSB-EP467300ZAX
CSB-BP467300ZAX
CSB-MP467300ZAX
CSB-EP467300ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton tonsurans Metallocarboxypeptidase A (MCPA) CSB-YP467301TQB
CSB-EP467301TQB
CSB-BP467301TQB
CSB-MP467301TQB
CSB-EP467301TQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton tonsurans Extracellular metalloproteinase 2 (MEP2) CSB-YP467302TQB
CSB-EP467302TQB
CSB-BP467302TQB
CSB-MP467302TQB
CSB-EP467302TQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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