Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP467323DPP
CSB-EP467323DPP
CSB-BP467323DPP
CSB-MP467323DPP
CSB-EP467323DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable exosome complex exonuclease 1 (TON_0030) CSB-YP467324TMR
CSB-EP467324TMR
CSB-BP467324TMR
CSB-MP467324TMR
CSB-EP467324TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L2P (rpl2p) CSB-YP467325TMR
CSB-EP467325TMR
CSB-BP467325TMR
CSB-MP467325TMR
CSB-EP467325TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L6P (rpl6p) CSB-YP467326TMR
CSB-EP467326TMR
CSB-BP467326TMR
CSB-MP467326TMR
CSB-EP467326TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L14e (rpl14e) CSB-YP467327TMR
CSB-EP467327TMR
CSB-BP467327TMR
CSB-MP467327TMR
CSB-EP467327TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L40e (rpl40e) CSB-YP467328TMR
CSB-EP467328TMR
CSB-BP467328TMR
CSB-MP467328TMR
CSB-EP467328TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Leucine--tRNA ligase (leuS), partial CSB-YP467329TMR
CSB-EP467329TMR
CSB-BP467329TMR
CSB-MP467329TMR
CSB-EP467329TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus D-tyrosyl-tRNA (Tyr) deacylase CSB-YP467330TMR
CSB-EP467330TMR
CSB-BP467330TMR
CSB-MP467330TMR
CSB-EP467330TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Glutamate--tRNA ligase (gltX), partial CSB-YP467331TMR
CSB-EP467331TMR
CSB-BP467331TMR
CSB-MP467331TMR
CSB-EP467331TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-directed RNA polymerase subunit A' (rpoA2) CSB-YP467332TMR
CSB-EP467332TMR
CSB-BP467332TMR
CSB-MP467332TMR
CSB-EP467332TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Ribonuclease Z (rnz) CSB-YP467333TMR
CSB-EP467333TMR
CSB-BP467333TMR
CSB-MP467333TMR
CSB-EP467333TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S8e (rps8e) CSB-YP467334TMR
CSB-EP467334TMR
CSB-BP467334TMR
CSB-MP467334TMR
CSB-EP467334TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP467335TMR
CSB-EP467335TMR
CSB-BP467335TMR
CSB-MP467335TMR
CSB-EP467335TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Prefoldin subunit beta (pfdB) CSB-YP467336TMR
CSB-EP467336TMR
CSB-BP467336TMR
CSB-MP467336TMR
CSB-EP467336TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S3Ae (rps3Ae) CSB-YP467337TMR
CSB-EP467337TMR
CSB-BP467337TMR
CSB-MP467337TMR
CSB-EP467337TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP467338TMR
CSB-EP467338TMR
CSB-BP467338TMR
CSB-MP467338TMR
CSB-EP467338TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Cysteine--tRNA ligase (cysS) CSB-YP467339TMR
CSB-EP467339TMR
CSB-BP467339TMR
CSB-MP467339TMR
CSB-EP467339TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L37e (rpl37e) CSB-YP467340TMR
CSB-EP467340TMR
CSB-BP467340TMR
CSB-MP467340TMR
CSB-EP467340TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus V-type proton ATPase subunit E (atpE) CSB-YP467341TMR
CSB-EP467341TMR
CSB-BP467341TMR
CSB-MP467341TMR
CSB-EP467341TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable DNA primase small subunit (priA) CSB-YP467342TMR
CSB-EP467342TMR
CSB-BP467342TMR
CSB-MP467342TMR
CSB-EP467342TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>