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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Helicobacter pylori RNA pyrophosphohydrolase (rppH) CSB-YP467263HUY
CSB-EP467263HUY
CSB-BP467263HUY
CSB-MP467263HUY
CSB-EP467263HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Orotate phosphoribosyltransferase (pyrE) CSB-YP467264HUY
CSB-EP467264HUY
CSB-BP467264HUY
CSB-MP467264HUY
CSB-EP467264HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Anthranilate phosphoribosyltransferase (trpD) CSB-YP467265HUY
CSB-EP467265HUY
CSB-BP467265HUY
CSB-MP467265HUY
CSB-EP467265HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L6 (rplF) CSB-YP467266HUY
CSB-EP467266HUY
CSB-BP467266HUY
CSB-MP467266HUY
CSB-EP467266HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S17 (rpsQ) CSB-YP467267HUY
CSB-EP467267HUY
CSB-BP467267HUY
CSB-MP467267HUY
CSB-EP467267HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L4 (rplD) CSB-YP467268HUY
CSB-EP467268HUY
CSB-BP467268HUY
CSB-MP467268HUY
CSB-EP467268HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP467269HUY
CSB-EP467269HUY
CSB-BP467269HUY
CSB-MP467269HUY
CSB-EP467269HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP467270HUY
CSB-EP467270HUY
CSB-BP467270HUY
CSB-MP467270HUY
CSB-EP467270HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phosphopantetheine adenylyltransferase (coaD) CSB-YP467271HUY
CSB-EP467271HUY
CSB-BP467271HUY
CSB-MP467271HUY
CSB-EP467271HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori L-seryl-tRNA (Sec) selenium transferase CSB-YP467272HUY
CSB-EP467272HUY
CSB-BP467272HUY
CSB-MP467272HUY
CSB-EP467272HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Probable GTP-binding protein EngB (engB) CSB-YP467273HUY
CSB-EP467273HUY
CSB-BP467273HUY
CSB-MP467273HUY
CSB-EP467273HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Pantothenate synthetase (panC) CSB-YP467274HUY
CSB-EP467274HUY
CSB-BP467274HUY
CSB-MP467274HUY
CSB-EP467274HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease accessory protein UreG (ureG) CSB-YP467275HUY
CSB-EP467275HUY
CSB-BP467275HUY
CSB-MP467275HUY
CSB-EP467275HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 30S ribosomal protein S20 (rpsT) CSB-YP467276HUY
CSB-EP467276HUY
CSB-BP467276HUY
CSB-MP467276HUY
CSB-EP467276HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Protein grpE (grpE) CSB-YP467277HUY
CSB-EP467277HUY
CSB-BP467277HUY
CSB-MP467277HUY
CSB-EP467277HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor P (efp) CSB-YP467278HUY
CSB-EP467278HUY
CSB-BP467278HUY
CSB-MP467278HUY
CSB-EP467278HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Sister chromatid cohesion vasa, isoform solo (vas), partial CSB-YP467279DLU
CSB-EP467279DLU
CSB-BP467279DLU
CSB-MP467279DLU
CSB-EP467279DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus 40S ribosomal protein S1 (rps1) CSB-YP467280SXT
CSB-EP467280SXT
CSB-BP467280SXT
CSB-MP467280SXT
CSB-EP467280SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial, partial CSB-YP467281SXT
CSB-EP467281SXT
CSB-BP467281SXT
CSB-MP467281SXT
CSB-EP467281SXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces japonicus Protein sey1 (sey1), partial CSB-YP467282SXT1
CSB-EP467282SXT1
CSB-BP467282SXT1
CSB-MP467282SXT1
CSB-EP467282SXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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