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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L9 (rplI) CSB-YP468463AWI
CSB-EP468463AWI
CSB-BP468463AWI
CSB-MP468463AWI
CSB-EP468463AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable Fe (2+)-trafficking protein CSB-YP468464AWI
CSB-EP468464AWI
CSB-BP468464AWI
CSB-MP468464AWI
CSB-EP468464AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein RecA (recA) CSB-YP468465AWI
CSB-EP468465AWI
CSB-BP468465AWI
CSB-MP468465AWI
CSB-EP468465AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Aspartate carbamoyltransferase (pyrB) CSB-YP468466AWI
CSB-EP468466AWI
CSB-BP468466AWI
CSB-MP468466AWI
CSB-EP468466AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP468467AWI
CSB-EP468467AWI
CSB-BP468467AWI
CSB-MP468467AWI
CSB-EP468467AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans ATP synthase subunit c (atpE), partial CSB-YP468468AWI1
CSB-EP468468AWI1
CSB-BP468468AWI1
CSB-MP468468AWI1
CSB-EP468468AWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Thiamine-phosphate synthase (thiE) CSB-YP468469AWI
CSB-EP468469AWI
CSB-BP468469AWI
CSB-MP468469AWI
CSB-EP468469AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UPF0753 protein Lferr_1358 (Lferr_1358), partial CSB-YP468470AWI
CSB-EP468470AWI
CSB-BP468470AWI
CSB-MP468470AWI
CSB-EP468470AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Cobyric acid synthase (cobQ) CSB-YP468471VFC
CSB-EP468471VFC
CSB-BP468471VFC
CSB-MP468471VFC
CSB-EP468471VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0265 protein VFMJ11_A0615 (VFMJ11_A0615) CSB-YP468472VFC
CSB-EP468472VFC
CSB-BP468472VFC
CSB-MP468472VFC
CSB-EP468472VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Probable phosphatase VFMJ11_A0899 (VFMJ11_A0899) CSB-YP468473VFC
CSB-EP468473VFC
CSB-BP468473VFC
CSB-MP468473VFC
CSB-EP468473VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Sigma factor-binding protein crl (crl) CSB-YP468474SWK
CSB-EP468474SWK
CSB-BP468474SWK
CSB-MP468474SWK
CSB-EP468474SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP468475SWK
CSB-EP468475SWK
CSB-BP468475SWK
CSB-MP468475SWK
CSB-EP468475SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona E3 ubiquitin-protein ligase SopA (sopA), partial CSB-YP468476SWK
CSB-EP468476SWK
CSB-BP468476SWK
CSB-MP468476SWK
CSB-EP468476SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Xylose isomerase (xylA) CSB-YP468477SWK
CSB-EP468477SWK
CSB-BP468477SWK
CSB-MP468477SWK
CSB-EP468477SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP468478SWK
CSB-EP468478SWK
CSB-BP468478SWK
CSB-MP468478SWK
CSB-EP468478SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP468479SWK
CSB-EP468479SWK
CSB-BP468479SWK
CSB-MP468479SWK
CSB-EP468479SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0133 protein ybaB (ybaB) CSB-YP468480SWK
CSB-EP468480SWK
CSB-BP468480SWK
CSB-MP468480SWK
CSB-EP468480SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Methionine--tRNA ligase (metG), partial CSB-YP468481SWK
CSB-EP468481SWK
CSB-BP468481SWK
CSB-MP468481SWK
CSB-EP468481SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Putative transport protein YidE (yidE), partial CSB-YP468482SWK
CSB-EP468482SWK
CSB-BP468482SWK
CSB-MP468482SWK
CSB-EP468482SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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