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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter bemidjiensis FMN-dependent NADH-azoreductase (azoR) CSB-YP468443GFN
CSB-EP468443GFN
CSB-BP468443GFN
CSB-MP468443GFN
CSB-EP468443GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP468444GFN
CSB-EP468444GFN
CSB-BP468444GFN
CSB-MP468444GFN
CSB-EP468444GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP468445GFN
CSB-EP468445GFN
CSB-BP468445GFN
CSB-MP468445GFN
CSB-EP468445GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Peptidyl-tRNA hydrolase (pth) CSB-YP468446GFN
CSB-EP468446GFN
CSB-BP468446GFN
CSB-MP468446GFN
CSB-EP468446GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP468447GFN
CSB-EP468447GFN
CSB-BP468447GFN
CSB-MP468447GFN
CSB-EP468447GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP468448GFN
CSB-EP468448GFN
CSB-BP468448GFN
CSB-MP468448GFN
CSB-EP468448GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Recombination protein RecR (recR) CSB-YP468449AWI
CSB-EP468449AWI
CSB-BP468449AWI
CSB-MP468449AWI
CSB-EP468449AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Probable transcriptional regulatory protein Lferr_0060 (Lferr_0060) CSB-YP468450AWI
CSB-EP468450AWI
CSB-BP468450AWI
CSB-MP468450AWI
CSB-EP468450AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Tryptophan synthase beta chain (trpB) CSB-YP468451AWI
CSB-EP468451AWI
CSB-BP468451AWI
CSB-MP468451AWI
CSB-EP468451AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP468452AWI
CSB-EP468452AWI
CSB-BP468452AWI
CSB-MP468452AWI
CSB-EP468452AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L27 (rpmA) CSB-YP468453AWI
CSB-EP468453AWI
CSB-BP468453AWI
CSB-MP468453AWI
CSB-EP468453AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Transcriptional repressor NrdR (nrdR) CSB-YP468454AWI
CSB-EP468454AWI
CSB-BP468454AWI
CSB-MP468454AWI
CSB-EP468454AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S10 (rpsJ) CSB-YP468455AWI
CSB-EP468455AWI
CSB-BP468455AWI
CSB-MP468455AWI
CSB-EP468455AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L29 (rpmC) CSB-YP468456AWI
CSB-EP468456AWI
CSB-BP468456AWI
CSB-MP468456AWI
CSB-EP468456AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L30 (rpmD) CSB-YP468457AWI
CSB-EP468457AWI
CSB-BP468457AWI
CSB-MP468457AWI
CSB-EP468457AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Ribonuclease H (rnhA) CSB-YP468458AWI
CSB-EP468458AWI
CSB-BP468458AWI
CSB-MP468458AWI
CSB-EP468458AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP468459AWI
CSB-EP468459AWI
CSB-BP468459AWI
CSB-MP468459AWI
CSB-EP468459AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Chaperone protein DnaK (dnaK), partial CSB-YP468460AWI
CSB-EP468460AWI
CSB-BP468460AWI
CSB-MP468460AWI
CSB-EP468460AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Protein ApaG (apaG) CSB-YP468461AWI
CSB-EP468461AWI
CSB-BP468461AWI
CSB-MP468461AWI
CSB-EP468461AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP468462AWI
CSB-EP468462AWI
CSB-BP468462AWI
CSB-MP468462AWI
CSB-EP468462AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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