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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S13 (rpsM) CSB-YP469063TMY
CSB-EP469063TMY
CSB-BP469063TMY
CSB-MP469063TMY
CSB-EP469063TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 30S ribosomal protein S8 (rpsH) CSB-YP469064TMY
CSB-EP469064TMY
CSB-BP469064TMY
CSB-MP469064TMY
CSB-EP469064TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L23 (rplW) CSB-YP469065TMY
CSB-EP469065TMY
CSB-BP469065TMY
CSB-MP469065TMY
CSB-EP469065TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP469066TMY
CSB-EP469066TMY
CSB-BP469066TMY
CSB-MP469066TMY
CSB-EP469066TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Cytidylate kinase (cmk) CSB-YP469067TMY
CSB-EP469067TMY
CSB-BP469067TMY
CSB-MP469067TMY
CSB-EP469067TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Shikimate kinase (aroK) CSB-YP469068TMY
CSB-EP469068TMY
CSB-BP469068TMY
CSB-MP469068TMY
CSB-EP469068TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Adenylate kinase (adk) CSB-YP469069TMY
CSB-EP469069TMY
CSB-BP469069TMY
CSB-MP469069TMY
CSB-EP469069TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L9 (rplI) CSB-YP469070TMY
CSB-EP469070TMY
CSB-BP469070TMY
CSB-MP469070TMY
CSB-EP469070TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Release factor glutamine methyltransferase (prmC) CSB-YP469071TMY
CSB-EP469071TMY
CSB-BP469071TMY
CSB-MP469071TMY
CSB-EP469071TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Shikimate dehydrogenase (aroE) CSB-YP469072TMY
CSB-EP469072TMY
CSB-BP469072TMY
CSB-MP469072TMY
CSB-EP469072TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Pantothenate synthetase (panC) CSB-YP469073TMY
CSB-EP469073TMY
CSB-BP469073TMY
CSB-MP469073TMY
CSB-EP469073TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Transcription elongation factor GreA (greA) CSB-YP469074TMY
CSB-EP469074TMY
CSB-BP469074TMY
CSB-MP469074TMY
CSB-EP469074TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii DNA mismatch repair protein MutS (mutS), partial CSB-YP469075TMY
CSB-EP469075TMY
CSB-BP469075TMY
CSB-MP469075TMY
CSB-EP469075TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii 50S ribosomal protein L19 (rplS) CSB-YP469076TMY
CSB-EP469076TMY
CSB-BP469076TMY
CSB-MP469076TMY
CSB-EP469076TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP469077TMY
CSB-EP469077TMY
CSB-BP469077TMY
CSB-MP469077TMY
CSB-EP469077TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP469078TMY
CSB-EP469078TMY
CSB-BP469078TMY
CSB-MP469078TMY
CSB-EP469078TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Protein translocase subunit SecA (secA), partial CSB-YP469079TMY
CSB-EP469079TMY
CSB-BP469079TMY
CSB-MP469079TMY
CSB-EP469079TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii NADH-quinone oxidoreductase subunit N 2 (nuoN2), partial CSB-YP469080TMY
CSB-EP469080TMY
CSB-BP469080TMY
CSB-MP469080TMY
CSB-EP469080TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP469081TMY
CSB-EP469081TMY
CSB-BP469081TMY
CSB-MP469081TMY
CSB-EP469081TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermodesulfovibrio yellowstonii Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP469082TMY
CSB-EP469082TMY
CSB-BP469082TMY
CSB-MP469082TMY
CSB-EP469082TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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