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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Geobacter bemidjiensis LexA repressor (lexA) CSB-YP468423GFN
CSB-EP468423GFN
CSB-BP468423GFN
CSB-MP468423GFN
CSB-EP468423GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Probable endonuclease 4 (nfo) CSB-YP468424GFN
CSB-EP468424GFN
CSB-BP468424GFN
CSB-MP468424GFN
CSB-EP468424GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Glycerol kinase (glpK) CSB-YP468425GFN
CSB-EP468425GFN
CSB-BP468425GFN
CSB-MP468425GFN
CSB-EP468425GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis tRNA (guanine-N (1)-)-methyltransferase CSB-YP468426GFN
CSB-EP468426GFN
CSB-BP468426GFN
CSB-MP468426GFN
CSB-EP468426GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Glutamyl-tRNA reductase (hemA) CSB-YP468427GFN
CSB-EP468427GFN
CSB-BP468427GFN
CSB-MP468427GFN
CSB-EP468427GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis D-alanine--D-alanine ligase (ddl) CSB-YP468428GFN
CSB-EP468428GFN
CSB-BP468428GFN
CSB-MP468428GFN
CSB-EP468428GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP468429GFN
CSB-EP468429GFN
CSB-BP468429GFN
CSB-MP468429GFN
CSB-EP468429GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Quinolinate synthase A (nadA) CSB-YP468430GFN
CSB-EP468430GFN
CSB-BP468430GFN
CSB-MP468430GFN
CSB-EP468430GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP468431GFN
CSB-EP468431GFN
CSB-BP468431GFN
CSB-MP468431GFN
CSB-EP468431GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Lon protease (lon), partial CSB-YP468432GFN
CSB-EP468432GFN
CSB-BP468432GFN
CSB-MP468432GFN
CSB-EP468432GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Glutamate--tRNA ligase (gltX) CSB-YP468433GFN
CSB-EP468433GFN
CSB-BP468433GFN
CSB-MP468433GFN
CSB-EP468433GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Probable GTP-binding protein EngB (engB) CSB-YP468434GFN
CSB-EP468434GFN
CSB-BP468434GFN
CSB-MP468434GFN
CSB-EP468434GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP468435GFN
CSB-EP468435GFN
CSB-BP468435GFN
CSB-MP468435GFN
CSB-EP468435GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis ATP synthase epsilon chain (atpC) CSB-YP468436GFN
CSB-EP468436GFN
CSB-BP468436GFN
CSB-MP468436GFN
CSB-EP468436GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP468437GFN
CSB-EP468437GFN
CSB-BP468437GFN
CSB-MP468437GFN
CSB-EP468437GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 30S ribosomal protein S7 (rpsG) CSB-YP468438GFN
CSB-EP468438GFN
CSB-BP468438GFN
CSB-MP468438GFN
CSB-EP468438GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L22 (rplV) CSB-YP468439GFN
CSB-EP468439GFN
CSB-BP468439GFN
CSB-MP468439GFN
CSB-EP468439GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L5 (rplE) CSB-YP468440GFN
CSB-EP468440GFN
CSB-BP468440GFN
CSB-MP468440GFN
CSB-EP468440GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 30S ribosomal protein S13 (rpsM) CSB-YP468441GFN
CSB-EP468441GFN
CSB-BP468441GFN
CSB-MP468441GFN
CSB-EP468441GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis DNA mismatch repair protein mutL (mutL), partial CSB-YP468442GFN
CSB-EP468442GFN
CSB-BP468442GFN
CSB-MP468442GFN
CSB-EP468442GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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