Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 ATPase ravA (ravA) CSB-YP468703SWO
CSB-EP468703SWO
CSB-BP468703SWO
CSB-MP468703SWO
CSB-EP468703SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (wecG) CSB-YP468704SWO
CSB-EP468704SWO
CSB-BP468704SWO
CSB-MP468704SWO
CSB-EP468704SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0250 protein ybeD (ybeD) CSB-YP468705SWO
CSB-EP468705SWO
CSB-BP468705SWO
CSB-MP468705SWO
CSB-EP468705SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP468706SWO
CSB-EP468706SWO
CSB-BP468706SWO
CSB-MP468706SWO
CSB-EP468706SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP468707SWO
CSB-EP468707SWO
CSB-BP468707SWO
CSB-MP468707SWO
CSB-EP468707SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Adenylyl-sulfate kinase (cysC) CSB-YP468708SWO
CSB-EP468708SWO
CSB-BP468708SWO
CSB-MP468708SWO
CSB-EP468708SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP468709SWO1
CSB-EP468709SWO1
CSB-BP468709SWO1
CSB-MP468709SWO1
CSB-EP468709SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glucosamine-6-phosphate deaminase (nagB) CSB-YP468710SWO
CSB-EP468710SWO
CSB-BP468710SWO
CSB-MP468710SWO
CSB-EP468710SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 N-succinylarginine dihydrolase (astB) CSB-YP468711SWO
CSB-EP468711SWO
CSB-BP468711SWO
CSB-MP468711SWO
CSB-EP468711SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP468712SWO
CSB-EP468712SWO
CSB-BP468712SWO
CSB-MP468712SWO
CSB-EP468712SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 D-tyrosyl-tRNA (Tyr) deacylase CSB-YP468713SWO
CSB-EP468713SWO
CSB-BP468713SWO
CSB-MP468713SWO
CSB-EP468713SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional activator rhaS (rhaS) CSB-YP468714SWO
CSB-EP468714SWO
CSB-BP468714SWO
CSB-MP468714SWO
CSB-EP468714SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP468715SWO
CSB-EP468715SWO
CSB-BP468715SWO
CSB-MP468715SWO
CSB-EP468715SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Imidazolonepropionase (hutI) CSB-YP468716SWO
CSB-EP468716SWO
CSB-BP468716SWO
CSB-MP468716SWO
CSB-EP468716SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP468717SWO
CSB-EP468717SWO
CSB-BP468717SWO
CSB-MP468717SWO
CSB-EP468717SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Thiamine kinase (thiK) CSB-YP468718SWO
CSB-EP468718SWO
CSB-BP468718SWO
CSB-MP468718SWO
CSB-EP468718SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Lysine--tRNA ligase (lysS), partial CSB-YP468719SWO
CSB-EP468719SWO
CSB-BP468719SWO
CSB-MP468719SWO
CSB-EP468719SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0149 protein ygfB (ygfB) CSB-YP468720SWO
CSB-EP468720SWO
CSB-BP468720SWO
CSB-MP468720SWO
CSB-EP468720SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 ATP-dependent protease subunit HslV (hslV) CSB-YP468721SWO
CSB-EP468721SWO
CSB-BP468721SWO
CSB-MP468721SWO
CSB-EP468721SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Pantothenate kinase (coaA) CSB-YP468722SWO
CSB-EP468722SWO
CSB-BP468722SWO
CSB-MP468722SWO
CSB-EP468722SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>