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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) CSB-YP468783SWP
CSB-EP468783SWP
CSB-BP468783SWP
CSB-MP468783SWP
CSB-EP468783SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP468784SWP
CSB-EP468784SWP
CSB-BP468784SWP
CSB-MP468784SWP
CSB-EP468784SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Acyl carrier protein phosphodiesterase (acpH) CSB-YP468785SWP
CSB-EP468785SWP
CSB-BP468785SWP
CSB-MP468785SWP
CSB-EP468785SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP468786SWP
CSB-EP468786SWP
CSB-BP468786SWP
CSB-MP468786SWP
CSB-EP468786SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP468787SWP
CSB-EP468787SWP
CSB-BP468787SWP
CSB-MP468787SWP
CSB-EP468787SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Bifunctional protein FolD (folD) CSB-YP468788SWP
CSB-EP468788SWP
CSB-BP468788SWP
CSB-MP468788SWP
CSB-EP468788SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP468789SWP
CSB-EP468789SWP
CSB-BP468789SWP
CSB-MP468789SWP
CSB-EP468789SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Flagellar protein FliT (fliT) CSB-YP468790SWP
CSB-EP468790SWP
CSB-BP468790SWP
CSB-MP468790SWP
CSB-EP468790SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Peptidase E (pepE) CSB-YP468791SWP
CSB-EP468791SWP
CSB-BP468791SWP
CSB-MP468791SWP
CSB-EP468791SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP468792SWP
CSB-EP468792SWP
CSB-BP468792SWP
CSB-MP468792SWP
CSB-EP468792SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP468793SWP
CSB-EP468793SWP
CSB-BP468793SWP
CSB-MP468793SWP
CSB-EP468793SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Cytidylate kinase (cmk) CSB-YP468794SWP
CSB-EP468794SWP
CSB-BP468794SWP
CSB-MP468794SWP
CSB-EP468794SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Dihydroorotate dehydrogenase (quinone) CSB-YP468795SWP
CSB-EP468795SWP
CSB-BP468795SWP
CSB-MP468795SWP
CSB-EP468795SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Fatty acid metabolism regulator protein (fadR) CSB-YP468796SWP
CSB-EP468796SWP
CSB-BP468796SWP
CSB-MP468796SWP
CSB-EP468796SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Cation/acetate symporter ActP (actP), partial CSB-YP468797SWP
CSB-EP468797SWP
CSB-BP468797SWP
CSB-MP468797SWP
CSB-EP468797SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Elongation factor P (efp) CSB-YP468798SWP
CSB-EP468798SWP
CSB-BP468798SWP
CSB-MP468798SWP
CSB-EP468798SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein hfq (hfq) CSB-YP468799SWP
CSB-EP468799SWP
CSB-BP468799SWP
CSB-MP468799SWP
CSB-EP468799SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum L-ribulose-5-phosphate 3-epimerase UlaE (ulaE) CSB-YP468800SWP
CSB-EP468800SWP
CSB-BP468800SWP
CSB-MP468800SWP
CSB-EP468800SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Aspartate carbamoyltransferase (pyrB) CSB-YP468801SWP
CSB-EP468801SWP
CSB-BP468801SWP
CSB-MP468801SWP
CSB-EP468801SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0060 membrane protein ynfA (ynfA), partial CSB-YP468802SWP1
CSB-EP468802SWP1
CSB-BP468802SWP1
CSB-MP468802SWP1
CSB-EP468802SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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