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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP468763SWO
CSB-EP468763SWO
CSB-BP468763SWO
CSB-MP468763SWO
CSB-EP468763SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP468764SWO
CSB-EP468764SWO
CSB-BP468764SWO
CSB-MP468764SWO
CSB-EP468764SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Outer-membrane lipoprotein lolB (lolB) CSB-YP468765SWO
CSB-EP468765SWO
CSB-BP468765SWO
CSB-MP468765SWO
CSB-EP468765SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0225 protein ychJ (ychJ) CSB-YP468766SWO
CSB-EP468766SWO
CSB-BP468766SWO
CSB-MP468766SWO
CSB-EP468766SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Sulfurtransferase TusA (tusA) CSB-YP468767SWO
CSB-EP468767SWO
CSB-BP468767SWO
CSB-MP468767SWO
CSB-EP468767SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP468768SWO
CSB-EP468768SWO
CSB-BP468768SWO
CSB-MP468768SWO
CSB-EP468768SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ribonuclease HII (rnhB) CSB-YP468769SWO
CSB-EP468769SWO
CSB-BP468769SWO
CSB-MP468769SWO
CSB-EP468769SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0283 membrane protein ycjF (ycjF), partial CSB-YP468770SWO1
CSB-EP468770SWO1
CSB-BP468770SWO1
CSB-MP468770SWO1
CSB-EP468770SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0370 protein ypfN (ypfN), partial CSB-YP468771SWO
CSB-EP468771SWO
CSB-BP468771SWO
CSB-MP468771SWO
CSB-EP468771SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Esterase FrsA (frsA) CSB-YP468772SWO
CSB-EP468772SWO
CSB-BP468772SWO
CSB-MP468772SWO
CSB-EP468772SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 GTPase Der (der) CSB-YP468773SWO
CSB-EP468773SWO
CSB-BP468773SWO
CSB-MP468773SWO
CSB-EP468773SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Iron-binding protein IscA (iscA) CSB-YP468774SWO
CSB-EP468774SWO
CSB-BP468774SWO
CSB-MP468774SWO
CSB-EP468774SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP468775SWO
CSB-EP468775SWO
CSB-BP468775SWO
CSB-MP468775SWO
CSB-EP468775SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0758 protein yicR (yicR) CSB-YP468776SWO
CSB-EP468776SWO
CSB-BP468776SWO
CSB-MP468776SWO
CSB-EP468776SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Chaperone protein DnaJ (dnaJ) CSB-YP468777SWO
CSB-EP468777SWO
CSB-BP468777SWO
CSB-MP468777SWO
CSB-EP468777SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Xanthine phosphoribosyltransferase (gpt) CSB-YP468778SWP
CSB-EP468778SWP
CSB-BP468778SWP
CSB-MP468778SWP
CSB-EP468778SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0345 protein YaiE (yaiE) CSB-YP468779SWP
CSB-EP468779SWP
CSB-BP468779SWP
CSB-MP468779SWP
CSB-EP468779SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Potassium-transporting ATPase B chain (kdpB), partial CSB-YP468780SWP1
CSB-EP468780SWP1
CSB-BP468780SWP1
CSB-MP468780SWP1
CSB-EP468780SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein TolB (tolB) CSB-YP468781SWP
CSB-EP468781SWP
CSB-BP468781SWP
CSB-MP468781SWP
CSB-EP468781SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Cell division inhibitor SulA (sulA) CSB-YP468782SWP
CSB-EP468782SWP
CSB-BP468782SWP
CSB-MP468782SWP
CSB-EP468782SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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