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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella enteritidis PT4 HTH-type transcriptional repressor NsrR (nsrR) CSB-YP468743SWO
CSB-EP468743SWO
CSB-BP468743SWO
CSB-MP468743SWO
CSB-EP468743SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Iron-sulfur cluster repair protein YtfE (ytfE) CSB-YP468744SWO
CSB-EP468744SWO
CSB-BP468744SWO
CSB-MP468744SWO
CSB-EP468744SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 tRNA (mo5U34)-methyltransferase CSB-YP468745SWO
CSB-EP468745SWO
CSB-BP468745SWO
CSB-MP468745SWO
CSB-EP468745SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Probable endonuclease 4 (nfo) CSB-YP468746SWO
CSB-EP468746SWO
CSB-BP468746SWO
CSB-MP468746SWO
CSB-EP468746SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP468747SWO1
CSB-EP468747SWO1
CSB-BP468747SWO1
CSB-MP468747SWO1
CSB-EP468747SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 DNA-binding protein fis (fis) CSB-YP468748SWO
CSB-EP468748SWO
CSB-BP468748SWO
CSB-MP468748SWO
CSB-EP468748SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Aspartate carbamoyltransferase (pyrB) CSB-YP468749SWO
CSB-EP468749SWO
CSB-BP468749SWO
CSB-MP468749SWO
CSB-EP468749SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein ApaG (apaG) CSB-YP468750SWO
CSB-EP468750SWO
CSB-BP468750SWO
CSB-MP468750SWO
CSB-EP468750SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP468751SWO
CSB-EP468751SWO
CSB-BP468751SWO
CSB-MP468751SWO
CSB-EP468751SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP468752SWO
CSB-EP468752SWO
CSB-BP468752SWO
CSB-MP468752SWO
CSB-EP468752SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP468753SWO1
CSB-EP468753SWO1
CSB-BP468753SWO1
CSB-MP468753SWO1
CSB-EP468753SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 50S ribosomal protein L16 (rplP) CSB-YP468754SWO
CSB-EP468754SWO
CSB-BP468754SWO
CSB-MP468754SWO
CSB-EP468754SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 50S ribosomal protein L3 (rplC) CSB-YP468755SWO
CSB-EP468755SWO
CSB-BP468755SWO
CSB-MP468755SWO
CSB-EP468755SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein TusC (tusC) CSB-YP468756SWO
CSB-EP468756SWO
CSB-BP468756SWO
CSB-MP468756SWO
CSB-EP468756SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 3-dehydroquinate synthase (aroB) CSB-YP468757SWO
CSB-EP468757SWO
CSB-BP468757SWO
CSB-MP468757SWO
CSB-EP468757SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Peptide chain release factor 3 (prfC), partial CSB-YP468758SWO
CSB-EP468758SWO
CSB-BP468758SWO
CSB-MP468758SWO
CSB-EP468758SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein MraZ (mraZ) CSB-YP468759SWO
CSB-EP468759SWO
CSB-BP468759SWO
CSB-MP468759SWO
CSB-EP468759SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0289 protein yacF (yacF) CSB-YP468760SWO
CSB-EP468760SWO
CSB-BP468760SWO
CSB-MP468760SWO
CSB-EP468760SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0181 protein yoaH (yoaH) CSB-YP468761SWO
CSB-EP468761SWO
CSB-BP468761SWO
CSB-MP468761SWO
CSB-EP468761SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Cell volume regulation protein A (cvrA), partial CSB-YP468762SWO
CSB-EP468762SWO
CSB-BP468762SWO
CSB-MP468762SWO
CSB-EP468762SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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