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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii Probable transcriptional regulatory protein CbuK_1793 (CbuK_1793) CSB-YP469523DXN
CSB-EP469523DXN
CSB-BP469523DXN
CSB-MP469523DXN
CSB-EP469523DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glutamate--tRNA ligase 1 (gltX1) CSB-YP469524DXN
CSB-EP469524DXN
CSB-BP469524DXN
CSB-MP469524DXN
CSB-EP469524DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S3 (rpsC) CSB-YP469525DXN
CSB-EP469525DXN
CSB-BP469525DXN
CSB-MP469525DXN
CSB-EP469525DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L14 (rplN) CSB-YP469526DXN
CSB-EP469526DXN
CSB-BP469526DXN
CSB-MP469526DXN
CSB-EP469526DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP469527DXN
CSB-EP469527DXN
CSB-BP469527DXN
CSB-MP469527DXN
CSB-EP469527DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L28 (rpmB) CSB-YP469528DXN
CSB-EP469528DXN
CSB-BP469528DXN
CSB-MP469528DXN
CSB-EP469528DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP469529DXN
CSB-EP469529DXN
CSB-BP469529DXN
CSB-MP469529DXN
CSB-EP469529DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP469530DXN
CSB-EP469530DXN
CSB-BP469530DXN
CSB-MP469530DXN
CSB-EP469530DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii S-adenosylmethionine synthase (metK) CSB-YP469531DXN
CSB-EP469531DXN
CSB-BP469531DXN
CSB-MP469531DXN
CSB-EP469531DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP469532DXN
CSB-EP469532DXN
CSB-BP469532DXN
CSB-MP469532DXN
CSB-EP469532DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Alanine racemase (alr) CSB-YP469533DXN
CSB-EP469533DXN
CSB-BP469533DXN
CSB-MP469533DXN
CSB-EP469533DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Adenylosuccinate synthetase (purA) CSB-YP469534DXN
CSB-EP469534DXN
CSB-BP469534DXN
CSB-MP469534DXN
CSB-EP469534DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Integration host factor subunit alpha (ihfA) CSB-YP469535DXN
CSB-EP469535DXN
CSB-BP469535DXN
CSB-MP469535DXN
CSB-EP469535DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphoglucosamine mutase (glmM) CSB-YP469536DXN
CSB-EP469536DXN
CSB-BP469536DXN
CSB-MP469536DXN
CSB-EP469536DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable rRNA maturation factor (CbuK_1270) CSB-YP469537DXN
CSB-EP469537DXN
CSB-BP469537DXN
CSB-MP469537DXN
CSB-EP469537DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP469538DXN
CSB-EP469538DXN
CSB-BP469538DXN
CSB-MP469538DXN
CSB-EP469538DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphate acyltransferase (plsX) CSB-YP469539DXN
CSB-EP469539DXN
CSB-BP469539DXN
CSB-MP469539DXN
CSB-EP469539DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L19 (rplS) CSB-YP469540DXN
CSB-EP469540DXN
CSB-BP469540DXN
CSB-MP469540DXN
CSB-EP469540DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Transcriptional repressor NrdR (nrdR) CSB-YP469541DXN
CSB-EP469541DXN
CSB-BP469541DXN
CSB-MP469541DXN
CSB-EP469541DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Membrane protein insertase YidC (yidC), partial CSB-YP469542DXN1
CSB-EP469542DXN1
CSB-BP469542DXN1
CSB-MP469542DXN1
CSB-EP469542DXN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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