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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Deoxyribose-phosphate aldolase (deoC) CSB-YP470123BQM
CSB-EP470123BQM
CSB-BP470123BQM
CSB-MP470123BQM
CSB-EP470123BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP470124BQM
CSB-EP470124BQM
CSB-BP470124BQM
CSB-MP470124BQM
CSB-EP470124BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0296 protein BCAH820_3886 (BCAH820_3886) CSB-YP470125BQM
CSB-EP470125BQM
CSB-BP470125BQM
CSB-MP470125BQM
CSB-EP470125BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotase (pyrC) CSB-YP470126BQM
CSB-EP470126BQM
CSB-BP470126BQM
CSB-MP470126BQM
CSB-EP470126BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lipoprotein signal peptidase (lspA), partial CSB-YP470127BQM1
CSB-EP470127BQM1
CSB-BP470127BQM1
CSB-MP470127BQM1
CSB-EP470127BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP470128BQM
CSB-EP470128BQM
CSB-BP470128BQM
CSB-MP470128BQM
CSB-EP470128BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Heme A synthase (ctaA), partial CSB-YP470129BQM
CSB-EP470129BQM
CSB-BP470129BQM
CSB-MP470129BQM
CSB-EP470129BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Type III pantothenate kinase (coaX) CSB-YP470130BQM
CSB-EP470130BQM
CSB-BP470130BQM
CSB-MP470130BQM
CSB-EP470130BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP470131BQM
CSB-EP470131BQM
CSB-BP470131BQM
CSB-MP470131BQM
CSB-EP470131BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L7/L12 (rplL) CSB-YP470132BQM
CSB-EP470132BQM
CSB-BP470132BQM
CSB-MP470132BQM
CSB-EP470132BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L3 (rplC) CSB-YP470133BQM
CSB-EP470133BQM
CSB-BP470133BQM
CSB-MP470133BQM
CSB-EP470133BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L16 (rplP) CSB-YP470134BQM
CSB-EP470134BQM
CSB-BP470134BQM
CSB-MP470134BQM
CSB-EP470134BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S8 (rpsH) CSB-YP470135BQM
CSB-EP470135BQM
CSB-BP470135BQM
CSB-MP470135BQM
CSB-EP470135BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L36 (rpmJ) CSB-YP470136BQM
CSB-EP470136BQM
CSB-BP470136BQM
CSB-MP470136BQM
CSB-EP470136BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Homoserine kinase (thrB) CSB-YP470137BQM
CSB-EP470137BQM
CSB-BP470137BQM
CSB-MP470137BQM
CSB-EP470137BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Metallothiol transferase fosB (fosB) CSB-YP470138BQM
CSB-EP470138BQM
CSB-BP470138BQM
CSB-MP470138BQM
CSB-EP470138BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP470139BQM
CSB-EP470139BQM
CSB-BP470139BQM
CSB-MP470139BQM
CSB-EP470139BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP470140BQM
CSB-EP470140BQM
CSB-BP470140BQM
CSB-MP470140BQM
CSB-EP470140BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglucosamine mutase (glmM) CSB-YP470141BQM
CSB-EP470141BQM
CSB-BP470141BQM
CSB-MP470141BQM
CSB-EP470141BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP470142BQM
CSB-EP470142BQM
CSB-BP470142BQM
CSB-MP470142BQM
CSB-EP470142BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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