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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Penicillium chrysogenum Transcriptional activator of proteases prtT (prtT), partial CSB-YP469363ETH
CSB-EP469363ETH
CSB-BP469363ETH
CSB-MP469363ETH
CSB-EP469363ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Long chronological lifespan protein 2 (lcl2) CSB-YP469364ETH
CSB-EP469364ETH
CSB-BP469364ETH
CSB-MP469364ETH
CSB-EP469364ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase C chain (kdpC), partial CSB-YP469365ENW1
CSB-EP469365ENW1
CSB-BP469365ENW1
CSB-MP469365ENW1
CSB-EP469365ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-tagatose-1,6-bisphosphate aldolase subunit gatZ (gatZ) CSB-YP469366ENW
CSB-EP469366ENW
CSB-BP469366ENW
CSB-MP469366ENW
CSB-EP469366ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable crotonobetaine/carnitine-CoA ligase (caiC) CSB-YP469367ENW
CSB-EP469367ENW
CSB-BP469367ENW
CSB-MP469367ENW
CSB-EP469367ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Homoserine kinase (thrB) CSB-YP469368ENW
CSB-EP469368ENW
CSB-BP469368ENW
CSB-MP469368ENW
CSB-EP469368ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipoprotein signal peptidase (lspA), partial CSB-YP469369ENW1
CSB-EP469369ENW1
CSB-BP469369ENW1
CSB-MP469369ENW1
CSB-EP469369ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP469370ENW
CSB-EP469370ENW
CSB-BP469370ENW
CSB-MP469370ENW
CSB-EP469370ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP469371ENW
CSB-EP469371ENW
CSB-BP469371ENW
CSB-MP469371ENW
CSB-EP469371ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0231 protein yacL (yacL) CSB-YP469372ENW
CSB-EP469372ENW
CSB-BP469372ENW
CSB-MP469372ENW
CSB-EP469372ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP469373ENW
CSB-EP469373ENW
CSB-BP469373ENW
CSB-MP469373ENW
CSB-EP469373ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP469374ENW
CSB-EP469374ENW
CSB-BP469374ENW
CSB-MP469374ENW
CSB-EP469374ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anti-adapter protein iraP (iraP) CSB-YP469375ENW
CSB-EP469375ENW
CSB-BP469375ENW
CSB-MP469375ENW
CSB-EP469375ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP469376ENW
CSB-EP469376ENW
CSB-BP469376ENW
CSB-MP469376ENW
CSB-EP469376ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA sulfurtransferase (thiI) CSB-YP469377ENW
CSB-EP469377ENW
CSB-BP469377ENW
CSB-MP469377ENW
CSB-EP469377ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribonuclease H (rnhA) CSB-YP469378ENW
CSB-EP469378ENW
CSB-BP469378ENW
CSB-MP469378ENW
CSB-EP469378ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Xanthine phosphoribosyltransferase (gpt) CSB-YP469379ENW
CSB-EP469379ENW
CSB-BP469379ENW
CSB-MP469379ENW
CSB-EP469379ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0386 protein yjhX (yjhX) CSB-YP469380ENW
CSB-EP469380ENW
CSB-BP469380ENW
CSB-MP469380ENW
CSB-EP469380ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP469381ENW
CSB-EP469381ENW
CSB-BP469381ENW
CSB-MP469381ENW
CSB-EP469381ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Citrate lyase acyl carrier protein (citD) CSB-YP469382ENW
CSB-EP469382ENW
CSB-BP469382ENW
CSB-MP469382ENW
CSB-EP469382ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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