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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Acinetobacter baumannii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP469783AWN
CSB-EP469783AWN
CSB-BP469783AWN
CSB-MP469783AWN
CSB-EP469783AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UPF0271 protein ABBFA_002265 (ABBFA_002265) CSB-YP469784AWN
CSB-EP469784AWN
CSB-BP469784AWN
CSB-MP469784AWN
CSB-EP469784AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP469785BQO
CSB-EP469785BQO
CSB-BP469785BQO
CSB-MP469785BQO
CSB-EP469785BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Sec-independent protein translocase protein TatA (tatA), partial CSB-YP469786BQO
CSB-EP469786BQO
CSB-BP469786BQO
CSB-MP469786BQO
CSB-EP469786BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable malate:quinone oxidoreductase (mqo) CSB-YP469787BQO
CSB-EP469787BQO
CSB-BP469787BQO
CSB-MP469787BQO
CSB-EP469787BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Orotate phosphoribosyltransferase (pyrE) CSB-YP469788BQO
CSB-EP469788BQO
CSB-BP469788BQO
CSB-MP469788BQO
CSB-EP469788BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional protein pyrR (pyrR) CSB-YP469789BQO
CSB-EP469789BQO
CSB-BP469789BQO
CSB-MP469789BQO
CSB-EP469789BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized N-acetyltransferase BCB4264_A4022 (BCB4264_A4022) CSB-YP469790BQO
CSB-EP469790BQO
CSB-BP469790BQO
CSB-MP469790BQO
CSB-EP469790BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0637 protein BCB4264_A4063 (BCB4264_A4063) CSB-YP469791BQO
CSB-EP469791BQO
CSB-BP469791BQO
CSB-MP469791BQO
CSB-EP469791BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0223 protein BCB4264_A4064 (BCB4264_A4064) CSB-YP469792BQO
CSB-EP469792BQO
CSB-BP469792BQO
CSB-MP469792BQO
CSB-EP469792BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0435 protein BCB4264_A0471 (BCB4264_A0471) CSB-YP469793BQO
CSB-EP469793BQO
CSB-BP469793BQO
CSB-MP469793BQO
CSB-EP469793BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0061 protein BCB4264_A3508 (BCB4264_A3508) CSB-YP469794BQO
CSB-EP469794BQO
CSB-BP469794BQO
CSB-MP469794BQO
CSB-EP469794BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP469795BQO
CSB-EP469795BQO
CSB-BP469795BQO
CSB-MP469795BQO
CSB-EP469795BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anti-sigma F factor (spoIIAB) CSB-YP469796BQO
CSB-EP469796BQO
CSB-BP469796BQO
CSB-MP469796BQO
CSB-EP469796BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus S-ribosylhomocysteine lyase (luxS) CSB-YP469797BQO
CSB-EP469797BQO
CSB-BP469797BQO
CSB-MP469797BQO
CSB-EP469797BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable manganese-dependent inorganic pyrophosphatase (ppaC) CSB-YP469798BQO
CSB-EP469798BQO
CSB-BP469798BQO
CSB-MP469798BQO
CSB-EP469798BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein N (sspN) CSB-YP469799BQO
CSB-EP469799BQO
CSB-BP469799BQO
CSB-MP469799BQO
CSB-EP469799BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N utilization substance protein B homolog (nusB) CSB-YP469800BQO
CSB-EP469800BQO
CSB-BP469800BQO
CSB-MP469800BQO
CSB-EP469800BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP469801BQO
CSB-EP469801BQO
CSB-BP469801BQO
CSB-MP469801BQO
CSB-EP469801BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazolonepropionase (hutI) CSB-YP469802BQO
CSB-EP469802BQO
CSB-BP469802BQO
CSB-MP469802BQO
CSB-EP469802BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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