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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP469843BQL
CSB-EP469843BQL
CSB-BP469843BQL
CSB-MP469843BQL
CSB-EP469843BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP469844BQL
CSB-EP469844BQL
CSB-BP469844BQL
CSB-MP469844BQL
CSB-EP469844BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holin-like protein CidA (cidA), partial CSB-YP469845BQL1
CSB-EP469845BQL1
CSB-BP469845BQL1
CSB-MP469845BQL1
CSB-EP469845BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cytidylate kinase (cmk) CSB-YP469846BQL
CSB-EP469846BQL
CSB-BP469846BQL
CSB-MP469846BQL
CSB-EP469846BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Demethylmenaquinone methyltransferase (ubiE) CSB-YP469847BQL
CSB-EP469847BQL
CSB-BP469847BQL
CSB-MP469847BQL
CSB-EP469847BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus CCA-adding enzyme (cca) CSB-YP469848BQL
CSB-EP469848BQL
CSB-BP469848BQL
CSB-MP469848BQL
CSB-EP469848BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Xanthine phosphoribosyltransferase (xpt) CSB-YP469849BQL
CSB-EP469849BQL
CSB-BP469849BQL
CSB-MP469849BQL
CSB-EP469849BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S15 (rpsO) CSB-YP469850BQL
CSB-EP469850BQL
CSB-BP469850BQL
CSB-MP469850BQL
CSB-EP469850BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent protease subunit HslV (hslV) CSB-YP469851BQL
CSB-EP469851BQL
CSB-BP469851BQL
CSB-MP469851BQL
CSB-EP469851BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit CSB-YP469852BQL
CSB-EP469852BQL
CSB-BP469852BQL
CSB-MP469852BQL
CSB-EP469852BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lipoprotein signal peptidase (lspA), partial CSB-YP469853BQL1
CSB-EP469853BQL1
CSB-BP469853BQL1
CSB-MP469853BQL1
CSB-EP469853BQL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP469854BQL
CSB-EP469854BQL
CSB-BP469854BQL
CSB-MP469854BQL
CSB-EP469854BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP469855BQL
CSB-EP469855BQL
CSB-BP469855BQL
CSB-MP469855BQL
CSB-EP469855BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP469856BQL
CSB-EP469856BQL
CSB-BP469856BQL
CSB-MP469856BQL
CSB-EP469856BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glucosamine-6-phosphate deaminase (nagB) CSB-YP469857BQL
CSB-EP469857BQL
CSB-BP469857BQL
CSB-MP469857BQL
CSB-EP469857BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Metallothiol transferase fosB (fosB) CSB-YP469858BQL
CSB-EP469858BQL
CSB-BP469858BQL
CSB-MP469858BQL
CSB-EP469858BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable butyrate kinase (buk) CSB-YP469859BQL
CSB-EP469859BQL
CSB-BP469859BQL
CSB-MP469859BQL
CSB-EP469859BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP469860BQL
CSB-EP469860BQL
CSB-BP469860BQL
CSB-MP469860BQL
CSB-EP469860BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate dehydratase (aroQ) CSB-YP469861BQL
CSB-EP469861BQL
CSB-BP469861BQL
CSB-MP469861BQL
CSB-EP469861BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP469862BQL
CSB-EP469862BQL
CSB-BP469862BQL
CSB-MP469862BQL
CSB-EP469862BQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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