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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP469803BQO
CSB-EP469803BQO
CSB-BP469803BQO
CSB-MP469803BQO
CSB-EP469803BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Germination protease (gpr) CSB-YP469804BQO
CSB-EP469804BQO
CSB-BP469804BQO
CSB-MP469804BQO
CSB-EP469804BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine--tRNA ligase (metG) CSB-YP469805BQO
CSB-EP469805BQO
CSB-BP469805BQO
CSB-MP469805BQO
CSB-EP469805BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Peptidase T (pepT) CSB-YP469806BQO
CSB-EP469806BQO
CSB-BP469806BQO
CSB-MP469806BQO
CSB-EP469806BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein RecA (recA) CSB-YP469807BQO
CSB-EP469807BQO
CSB-BP469807BQO
CSB-MP469807BQO
CSB-EP469807BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP469808BQO
CSB-EP469808BQO
CSB-BP469808BQO
CSB-MP469808BQO
CSB-EP469808BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S16 (rpsP) CSB-YP469809BQO
CSB-EP469809BQO
CSB-BP469809BQO
CSB-MP469809BQO
CSB-EP469809BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L28 (rpmB) CSB-YP469810BQO
CSB-EP469810BQO
CSB-BP469810BQO
CSB-MP469810BQO
CSB-EP469810BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0297 protein BCB4264_A4507 (BCB4264_A4507) CSB-YP469811BQO
CSB-EP469811BQO
CSB-BP469811BQO
CSB-MP469811BQO
CSB-EP469811BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP469812BQO
CSB-EP469812BQO
CSB-BP469812BQO
CSB-MP469812BQO
CSB-EP469812BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable septum site-determining protein MinC (minC) CSB-YP469813BQO
CSB-EP469813BQO
CSB-BP469813BQO
CSB-MP469813BQO
CSB-EP469813BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable GTP-binding protein EngB (engB) CSB-YP469814BQO
CSB-EP469814BQO
CSB-BP469814BQO
CSB-MP469814BQO
CSB-EP469814BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus SsrA-binding protein (smpB) CSB-YP469815BQO
CSB-EP469815BQO
CSB-BP469815BQO
CSB-MP469815BQO
CSB-EP469815BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein translocase subunit SecA (secA), partial CSB-YP469816BQO
CSB-EP469816BQO
CSB-BP469816BQO
CSB-MP469816BQO
CSB-EP469816BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0342 protein BCB4264_A0916 (BCB4264_A0916) CSB-YP469817BQO
CSB-EP469817BQO
CSB-BP469817BQO
CSB-MP469817BQO
CSB-EP469817BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L20 (rplT) CSB-YP469818BQO
CSB-EP469818BQO
CSB-BP469818BQO
CSB-MP469818BQO
CSB-EP469818BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP469819BQO
CSB-EP469819BQO
CSB-BP469819BQO
CSB-MP469819BQO
CSB-EP469819BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP synthase epsilon chain (atpC) CSB-YP469820BQO
CSB-EP469820BQO
CSB-BP469820BQO
CSB-MP469820BQO
CSB-EP469820BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3'-5' exoribonuclease yhaM (yhaM) CSB-YP469821BQO
CSB-EP469821BQO
CSB-BP469821BQO
CSB-MP469821BQO
CSB-EP469821BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Homoserine O-succinyltransferase (metA) CSB-YP469822BQO
CSB-EP469822BQO
CSB-BP469822BQO
CSB-MP469822BQO
CSB-EP469822BQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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