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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella agona Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP470883SWK
CSB-EP470883SWK
CSB-BP470883SWK
CSB-MP470883SWK
CSB-EP470883SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP470884SWK
CSB-EP470884SWK
CSB-BP470884SWK
CSB-MP470884SWK
CSB-EP470884SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0325 protein yaeH (yaeH) CSB-YP470885SWK
CSB-EP470885SWK
CSB-BP470885SWK
CSB-MP470885SWK
CSB-EP470885SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona UPF0294 protein yafD (yafD) CSB-YP470886SWK
CSB-EP470886SWK
CSB-BP470886SWK
CSB-MP470886SWK
CSB-EP470886SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Dihydroorotase (pyrC) CSB-YP470887SWK
CSB-EP470887SWK
CSB-BP470887SWK
CSB-MP470887SWK
CSB-EP470887SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0042 nucleotide-binding protein VFMJ11_0375 (VFMJ11_0375) CSB-YP470888VFC
CSB-EP470888VFC
CSB-BP470888VFC
CSB-MP470888VFC
CSB-EP470888VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Arginine deiminase (arcA) CSB-YP470889VFC
CSB-EP470889VFC
CSB-BP470889VFC
CSB-MP470889VFC
CSB-EP470889VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Putative Holliday junction resolvase (VFMJ11_0433) CSB-YP470890VFC
CSB-EP470890VFC
CSB-BP470890VFC
CSB-MP470890VFC
CSB-EP470890VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri RNA pyrophosphohydrolase (rppH) CSB-YP470891VFC
CSB-EP470891VFC
CSB-BP470891VFC
CSB-MP470891VFC
CSB-EP470891VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Lipid-A-disaccharide synthase (lpxB) CSB-YP470892VFC
CSB-EP470892VFC
CSB-BP470892VFC
CSB-MP470892VFC
CSB-EP470892VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Copper homeostasis protein CutC (cutC) CSB-YP470893VFC
CSB-EP470893VFC
CSB-BP470893VFC
CSB-MP470893VFC
CSB-EP470893VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP470894VFC1
CSB-EP470894VFC1
CSB-BP470894VFC1
CSB-MP470894VFC1
CSB-EP470894VFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri tRNA pseudouridine synthase B (truB) CSB-YP470895VFC
CSB-EP470895VFC
CSB-BP470895VFC
CSB-MP470895VFC
CSB-EP470895VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphopentomutase (deoB) CSB-YP470896VFC
CSB-EP470896VFC
CSB-BP470896VFC
CSB-MP470896VFC
CSB-EP470896VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri tRNA (guanine-N (1)-)-methyltransferase CSB-YP470897VFC
CSB-EP470897VFC
CSB-BP470897VFC
CSB-MP470897VFC
CSB-EP470897VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Cell division protein FtsB (ftsB), partial CSB-YP470898VFC
CSB-EP470898VFC
CSB-BP470898VFC
CSB-MP470898VFC
CSB-EP470898VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Autonomous glycyl radical cofactor (grcA) CSB-YP470899VFC
CSB-EP470899VFC
CSB-BP470899VFC
CSB-MP470899VFC
CSB-EP470899VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Sugar fermentation stimulation protein homolog (sfsA) CSB-YP470900VFC
CSB-EP470900VFC
CSB-BP470900VFC
CSB-MP470900VFC
CSB-EP470900VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Protein syd (syd) CSB-YP470901VFC
CSB-EP470901VFC
CSB-BP470901VFC
CSB-MP470901VFC
CSB-EP470901VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Histidine--tRNA ligase (hisS) CSB-YP470902VFC
CSB-EP470902VFC
CSB-BP470902VFC
CSB-MP470902VFC
CSB-EP470902VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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