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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes serotype M49 30S ribosomal protein S20 (rpsT) CSB-YP471283FNH
CSB-EP471283FNH
CSB-BP471283FNH
CSB-MP471283FNH
CSB-EP471283FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 GTPase obg (obg) CSB-YP471284FNH
CSB-EP471284FNH
CSB-BP471284FNH
CSB-MP471284FNH
CSB-EP471284FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Probable potassium transport system protein kup (kup), partial CSB-YP471285FNH
CSB-EP471285FNH
CSB-BP471285FNH
CSB-MP471285FNH
CSB-EP471285FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Bifunctional protein FolD (folD) CSB-YP471286FNH
CSB-EP471286FNH
CSB-BP471286FNH
CSB-MP471286FNH
CSB-EP471286FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP471287FNH
CSB-EP471287FNH
CSB-BP471287FNH
CSB-MP471287FNH
CSB-EP471287FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Shikimate dehydrogenase (aroE) CSB-YP471288FNH
CSB-EP471288FNH
CSB-BP471288FNH
CSB-MP471288FNH
CSB-EP471288FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Cell cycle protein GpsB (gpsB) CSB-YP471289FNH
CSB-EP471289FNH
CSB-BP471289FNH
CSB-MP471289FNH
CSB-EP471289FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP471290FNH
CSB-EP471290FNH
CSB-BP471290FNH
CSB-MP471290FNH
CSB-EP471290FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M49 Glycerol kinase (glpK), partial CSB-YP471291FNH
CSB-EP471291FNH
CSB-BP471291FNH
CSB-MP471291FNH
CSB-EP471291FNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae C4-dicarboxylate transport protein (dctA), partial CSB-YP471292KBH
CSB-EP471292KBH
CSB-BP471292KBH
CSB-MP471292KBH
CSB-EP471292KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Protein slyX (slyX) CSB-YP471293KBH
CSB-EP471293KBH
CSB-BP471293KBH
CSB-MP471293KBH
CSB-EP471293KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Sulfurtransferase TusD (tusD) CSB-YP471294KBH
CSB-EP471294KBH
CSB-BP471294KBH
CSB-MP471294KBH
CSB-EP471294KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L24 (rplX) CSB-YP471295KBH
CSB-EP471295KBH
CSB-BP471295KBH
CSB-MP471295KBH
CSB-EP471295KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 30S ribosomal protein S4 (rpsD) CSB-YP471296KBH
CSB-EP471296KBH
CSB-BP471296KBH
CSB-MP471296KBH
CSB-EP471296KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP471297KBH
CSB-EP471297KBH
CSB-BP471297KBH
CSB-MP471297KBH
CSB-EP471297KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae HTH-type transcriptional regulator iscR (iscR) CSB-YP471298KBH
CSB-EP471298KBH
CSB-BP471298KBH
CSB-MP471298KBH
CSB-EP471298KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Chaperone protein hscA (hscA), partial CSB-YP471299KBH
CSB-EP471299KBH
CSB-BP471299KBH
CSB-MP471299KBH
CSB-EP471299KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Histidine--tRNA ligase (hisS) CSB-YP471300KBH
CSB-EP471300KBH
CSB-BP471300KBH
CSB-MP471300KBH
CSB-EP471300KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Uracil phosphoribosyltransferase (upp) CSB-YP471301KBH
CSB-EP471301KBH
CSB-BP471301KBH
CSB-MP471301KBH
CSB-EP471301KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae UPF0207 protein KPK_1466 (KPK_1466) CSB-YP471302KBH
CSB-EP471302KBH
CSB-BP471302KBH
CSB-MP471302KBH
CSB-EP471302KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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