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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus 30S ribosomal protein S17 (rpsQ) CSB-YP472485BQM
CSB-EP472485BQM
CSB-BP472485BQM
CSB-MP472485BQM
CSB-EP472485BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L5 (rplE) CSB-YP472486BQM
CSB-EP472486BQM
CSB-BP472486BQM
CSB-MP472486BQM
CSB-EP472486BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Adenylate kinase (adk) CSB-YP472487BQM
CSB-EP472487BQM
CSB-BP472487BQM
CSB-MP472487BQM
CSB-EP472487BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L13 (rplM) CSB-YP472488BQM
CSB-EP472488BQM
CSB-BP472488BQM
CSB-MP472488BQM
CSB-EP472488BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0637 protein BCAH820_3975 (BCAH820_3975) CSB-YP472489BQM
CSB-EP472489BQM
CSB-BP472489BQM
CSB-MP472489BQM
CSB-EP472489BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP472490BQM
CSB-EP472490BQM
CSB-BP472490BQM
CSB-MP472490BQM
CSB-EP472490BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glucosamine-6-phosphate deaminase (nagB) CSB-YP472491BQM
CSB-EP472491BQM
CSB-BP472491BQM
CSB-MP472491BQM
CSB-EP472491BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP472492BQM
CSB-EP472492BQM
CSB-BP472492BQM
CSB-MP472492BQM
CSB-EP472492BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative 8-amino-7-oxononanoate synthase (bioF) CSB-YP472493BQM
CSB-EP472493BQM
CSB-BP472493BQM
CSB-MP472493BQM
CSB-EP472493BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP472494BQM
CSB-EP472494BQM
CSB-BP472494BQM
CSB-MP472494BQM
CSB-EP472494BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate dehydratase (aroQ) CSB-YP472495BQM
CSB-EP472495BQM
CSB-BP472495BQM
CSB-MP472495BQM
CSB-EP472495BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP472496BQM
CSB-EP472496BQM
CSB-BP472496BQM
CSB-MP472496BQM
CSB-EP472496BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA ligase (ligA), partial CSB-YP472497BQM
CSB-EP472497BQM
CSB-BP472497BQM
CSB-MP472497BQM
CSB-EP472497BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chromosome-anchoring protein racA (racA) CSB-YP472498BQM
CSB-EP472498BQM
CSB-BP472498BQM
CSB-MP472498BQM
CSB-EP472498BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable rRNA maturation factor (BCAH820_4325) CSB-YP472499BQM
CSB-EP472499BQM
CSB-BP472499BQM
CSB-MP472499BQM
CSB-EP472499BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaK (dnaK), partial CSB-YP472500BQM
CSB-EP472500BQM
CSB-BP472500BQM
CSB-MP472500BQM
CSB-EP472500BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein K (sspK) CSB-YP472501BQM
CSB-EP472501BQM
CSB-BP472501BQM
CSB-MP472501BQM
CSB-EP472501BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor 4 (lepA), partial CSB-YP472502BQM
CSB-EP472502BQM
CSB-BP472502BQM
CSB-MP472502BQM
CSB-EP472502BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP472503BQM
CSB-EP472503BQM
CSB-BP472503BQM
CSB-MP472503BQM
CSB-EP472503BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative Holliday junction resolvase (BCAH820_4466) CSB-YP472504BQM
CSB-EP472504BQM
CSB-BP472504BQM
CSB-MP472504BQM
CSB-EP472504BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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