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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida Protein CrcB homolog (crcB), partial CSB-YP471663AZM1
CSB-EP471663AZM1
CSB-BP471663AZM1
CSB-MP471663AZM1
CSB-EP471663AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP471664AZM
CSB-EP471664AZM
CSB-BP471664AZM
CSB-MP471664AZM
CSB-EP471664AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal protein S6 modification protein (rimK) CSB-YP471665AZM
CSB-EP471665AZM
CSB-BP471665AZM
CSB-MP471665AZM
CSB-EP471665AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Chaperone protein TorD (torD) CSB-YP471666AZM
CSB-EP471666AZM
CSB-BP471666AZM
CSB-MP471666AZM
CSB-EP471666AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Sec-independent protein translocase protein TatA (tatA), partial CSB-YP471667AZM
CSB-EP471667AZM
CSB-BP471667AZM
CSB-MP471667AZM
CSB-EP471667AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida ATP synthase gamma chain (atpG) CSB-YP471668AZM
CSB-EP471668AZM
CSB-BP471668AZM
CSB-MP471668AZM
CSB-EP471668AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Adenylate kinase (adk) CSB-YP471669AZM
CSB-EP471669AZM
CSB-BP471669AZM
CSB-MP471669AZM
CSB-EP471669AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Tyrosine--tRNA ligase (tyrS) CSB-YP471670AZM
CSB-EP471670AZM
CSB-BP471670AZM
CSB-MP471670AZM
CSB-EP471670AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Proline--tRNA ligase (proS), partial CSB-YP471671AZM
CSB-EP471671AZM
CSB-BP471671AZM
CSB-MP471671AZM
CSB-EP471671AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP471672AZM
CSB-EP471672AZM
CSB-BP471672AZM
CSB-MP471672AZM
CSB-EP471672AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP471673AZM
CSB-EP471673AZM
CSB-BP471673AZM
CSB-MP471673AZM
CSB-EP471673AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0061 protein VSAL_I2210 (VSAL_I2210) CSB-YP471674AZM
CSB-EP471674AZM
CSB-BP471674AZM
CSB-MP471674AZM
CSB-EP471674AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0352 protein VSAL_I1058 (VSAL_I1058) CSB-YP471675AZM
CSB-EP471675AZM
CSB-BP471675AZM
CSB-MP471675AZM
CSB-EP471675AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP471676AZM
CSB-EP471676AZM
CSB-BP471676AZM
CSB-MP471676AZM
CSB-EP471676AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phosphate acyltransferase (plsX) CSB-YP471677AZM
CSB-EP471677AZM
CSB-BP471677AZM
CSB-MP471677AZM
CSB-EP471677AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida ATP phosphoribosyltransferase (hisG) CSB-YP471678AZM
CSB-EP471678AZM
CSB-BP471678AZM
CSB-MP471678AZM
CSB-EP471678AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Tryptophan synthase beta chain (trpB) CSB-YP471679AZM
CSB-EP471679AZM
CSB-BP471679AZM
CSB-MP471679AZM
CSB-EP471679AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Cell division protein ZipA homolog (zipA), partial CSB-YP471680AZM
CSB-EP471680AZM
CSB-BP471680AZM
CSB-MP471680AZM
CSB-EP471680AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S2 (rpsB) CSB-YP471681AZM
CSB-EP471681AZM
CSB-BP471681AZM
CSB-MP471681AZM
CSB-EP471681AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida SsrA-binding protein (smpB) CSB-YP471682AZM
CSB-EP471682AZM
CSB-BP471682AZM
CSB-MP471682AZM
CSB-EP471682AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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