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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium leguminosarum bv. trifolii Gamma-glutamyl phosphate reductase (proA) CSB-YP471603RKQ
CSB-EP471603RKQ
CSB-BP471603RKQ
CSB-MP471603RKQ
CSB-EP471603RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Trans-aconitate 2-methyltransferase (tam) CSB-YP471604RKQ
CSB-EP471604RKQ
CSB-BP471604RKQ
CSB-MP471604RKQ
CSB-EP471604RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP471605RKQ
CSB-EP471605RKQ
CSB-BP471605RKQ
CSB-MP471605RKQ
CSB-EP471605RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP471606RKQ
CSB-EP471606RKQ
CSB-BP471606RKQ
CSB-MP471606RKQ
CSB-EP471606RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Malate synthase G (glcB), partial CSB-YP471607RKQ
CSB-EP471607RKQ
CSB-BP471607RKQ
CSB-MP471607RKQ
CSB-EP471607RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii SsrA-binding protein (smpB) CSB-YP471608RKQ
CSB-EP471608RKQ
CSB-BP471608RKQ
CSB-MP471608RKQ
CSB-EP471608RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Cysteine--tRNA ligase (cysS) CSB-YP471609RKQ
CSB-EP471609RKQ
CSB-BP471609RKQ
CSB-MP471609RKQ
CSB-EP471609RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii DNA ligase (ligA), partial CSB-YP471610RKQ
CSB-EP471610RKQ
CSB-BP471610RKQ
CSB-MP471610RKQ
CSB-EP471610RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Chaperone protein DnaJ (dnaJ) CSB-YP471611RKQ
CSB-EP471611RKQ
CSB-BP471611RKQ
CSB-MP471611RKQ
CSB-EP471611RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0303 protein Rleg2_2653 (Rleg2_2653) CSB-YP471612RKQ
CSB-EP471612RKQ
CSB-BP471612RKQ
CSB-MP471612RKQ
CSB-EP471612RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Adenine deaminase (Rleg2_4180) CSB-YP471613RKQ
CSB-EP471613RKQ
CSB-BP471613RKQ
CSB-MP471613RKQ
CSB-EP471613RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii N utilization substance protein B homolog (nusB) CSB-YP471614RKQ
CSB-EP471614RKQ
CSB-BP471614RKQ
CSB-MP471614RKQ
CSB-EP471614RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP471615RKQ
CSB-EP471615RKQ
CSB-BP471615RKQ
CSB-MP471615RKQ
CSB-EP471615RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP471616RKQ
CSB-EP471616RKQ
CSB-BP471616RKQ
CSB-MP471616RKQ
CSB-EP471616RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP471617RKQ
CSB-EP471617RKQ
CSB-BP471617RKQ
CSB-MP471617RKQ
CSB-EP471617RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP471618RKQ
CSB-EP471618RKQ
CSB-BP471618RKQ
CSB-MP471618RKQ
CSB-EP471618RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 50S ribosomal protein L1 (rplA) CSB-YP471619RKQ
CSB-EP471619RKQ
CSB-BP471619RKQ
CSB-MP471619RKQ
CSB-EP471619RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S10 (rpsJ) CSB-YP471620RKQ
CSB-EP471620RKQ
CSB-BP471620RKQ
CSB-MP471620RKQ
CSB-EP471620RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S3 (rpsC) CSB-YP471621RKQ
CSB-EP471621RKQ
CSB-BP471621RKQ
CSB-MP471621RKQ
CSB-EP471621RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 50S ribosomal protein L5 (rplE) CSB-YP471622RKQ
CSB-EP471622RKQ
CSB-BP471622RKQ
CSB-MP471622RKQ
CSB-EP471622RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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