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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Penicillium chrysogenum Probable Xaa-Pro aminopeptidase Pc16g13390 (Pc16g13390), partial CSB-YP471723ETH
CSB-EP471723ETH
CSB-BP471723ETH
CSB-MP471723ETH
CSB-EP471723ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Maintenance of mitochondrial morphology protein 1 (mmm1), partial CSB-YP471724ETH
CSB-EP471724ETH
CSB-BP471724ETH
CSB-MP471724ETH
CSB-EP471724ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Lipoyl synthase, mitochondrial (Pc20g03200) CSB-YP471725ETH
CSB-EP471725ETH
CSB-BP471725ETH
CSB-MP471725ETH
CSB-EP471725ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Altered inheritance of mitochondria protein 24, mitochondrial (aim24) CSB-YP471726ETH
CSB-EP471726ETH
CSB-BP471726ETH
CSB-MP471726ETH
CSB-EP471726ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum High osmolarity signaling protein sho1 (sho1), partial CSB-YP471727ETH1
CSB-EP471727ETH1
CSB-BP471727ETH1
CSB-MP471727ETH1
CSB-EP471727ETH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Cytosolic Fe-S cluster assembly factor nar1 (nar1), partial CSB-YP471728ETH
CSB-EP471728ETH
CSB-BP471728ETH
CSB-MP471728ETH
CSB-EP471728ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase B chain (kdpB), partial CSB-YP471729ENW1
CSB-EP471729ENW1
CSB-BP471729ENW1
CSB-MP471729ENW1
CSB-EP471729ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP471730ENW
CSB-EP471730ENW
CSB-BP471730ENW
CSB-MP471730ENW
CSB-EP471730ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Crotonobetainyl-CoA dehydrogenase (caiA) CSB-YP471731ENW
CSB-EP471731ENW
CSB-BP471731ENW
CSB-MP471731ENW
CSB-EP471731ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0412 protein yaaI (yaaI) CSB-YP471732ENW
CSB-EP471732ENW
CSB-BP471732ENW
CSB-MP471732ENW
CSB-EP471732ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutathione-regulated potassium-efflux system protein kefC (kefC), partial CSB-YP471733ENW
CSB-EP471733ENW
CSB-BP471733ENW
CSB-MP471733ENW
CSB-EP471733ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP471734ENW
CSB-EP471734ENW
CSB-BP471734ENW
CSB-MP471734ENW
CSB-EP471734ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein translocase subunit SecA (secA), partial CSB-YP471735ENW
CSB-EP471735ENW
CSB-BP471735ENW
CSB-MP471735ENW
CSB-EP471735ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pantothenate synthetase (panC) CSB-YP471736ENW
CSB-EP471736ENW
CSB-BP471736ENW
CSB-MP471736ENW
CSB-EP471736ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP471737ENW
CSB-EP471737ENW
CSB-BP471737ENW
CSB-MP471737ENW
CSB-EP471737ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S2 (rpsB) CSB-YP471738ENW
CSB-EP471738ENW
CSB-BP471738ENW
CSB-MP471738ENW
CSB-EP471738ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipid-A-disaccharide synthase (lpxB) CSB-YP471739ENW
CSB-EP471739ENW
CSB-BP471739ENW
CSB-MP471739ENW
CSB-EP471739ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0345 protein YaiE (yaiE) CSB-YP471740ENW
CSB-EP471740ENW
CSB-BP471740ENW
CSB-MP471740ENW
CSB-EP471740ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N utilization substance protein B homolog (nusB) CSB-YP471741ENW
CSB-EP471741ENW
CSB-BP471741ENW
CSB-MP471741ENW
CSB-EP471741ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 7-cyano-7-deazaguanine synthase (queC) CSB-YP471742ENW
CSB-EP471742ENW
CSB-BP471742ENW
CSB-MP471742ENW
CSB-EP471742ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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