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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermosipho africanus Bifunctional protein GlmU (glmU) CSB-YP472325TNG
CSB-EP472325TNG
CSB-BP472325TNG
CSB-MP472325TNG
CSB-EP472325TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus SsrA-binding protein (smpB) CSB-YP472326TNG
CSB-EP472326TNG
CSB-BP472326TNG
CSB-MP472326TNG
CSB-EP472326TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP472327TNG
CSB-EP472327TNG
CSB-BP472327TNG
CSB-MP472327TNG
CSB-EP472327TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Adenylosuccinate synthetase (purA) CSB-YP472328TNG
CSB-EP472328TNG
CSB-BP472328TNG
CSB-MP472328TNG
CSB-EP472328TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L1 (rplA) CSB-YP472329TNG
CSB-EP472329TNG
CSB-BP472329TNG
CSB-MP472329TNG
CSB-EP472329TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus GTPase obg (obg) CSB-YP472330TNG
CSB-EP472330TNG
CSB-BP472330TNG
CSB-MP472330TNG
CSB-EP472330TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Elongation factor P (efp) CSB-YP472331TNG
CSB-EP472331TNG
CSB-BP472331TNG
CSB-MP472331TNG
CSB-EP472331TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Cobalamin synthase (cobS), partial CSB-YP472332TNG1
CSB-EP472332TNG1
CSB-BP472332TNG1
CSB-MP472332TNG1
CSB-EP472332TNG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Histidine--tRNA ligase (hisS) CSB-YP472333TNG
CSB-EP472333TNG
CSB-BP472333TNG
CSB-MP472333TNG
CSB-EP472333TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S10 (rpsJ) CSB-YP472334TNG
CSB-EP472334TNG
CSB-BP472334TNG
CSB-MP472334TNG
CSB-EP472334TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 30S ribosomal protein S3 (rpsC) CSB-YP472335TNG
CSB-EP472335TNG
CSB-BP472335TNG
CSB-MP472335TNG
CSB-EP472335TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L5 (rplE) CSB-YP472336TNG
CSB-EP472336TNG
CSB-BP472336TNG
CSB-MP472336TNG
CSB-EP472336TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus 50S ribosomal protein L30 (rpmD) CSB-YP472337TNG
CSB-EP472337TNG
CSB-BP472337TNG
CSB-MP472337TNG
CSB-EP472337TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosipho africanus Adenylate kinase (adk) CSB-YP472338TNG
CSB-EP472338TNG
CSB-BP472338TNG
CSB-MP472338TNG
CSB-EP472338TNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0397 protein BCG9842_B2659 (BCG9842_B2659), partial CSB-YP472339BQP1
CSB-EP472339BQP1
CSB-BP472339BQP1
CSB-MP472339BQP1
CSB-EP472339BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L21 (rplU) CSB-YP472340BQP
CSB-EP472340BQP
CSB-BP472340BQP
CSB-MP472340BQP
CSB-EP472340BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease PH (rph) CSB-YP472341BQP
CSB-EP472341BQP
CSB-BP472341BQP
CSB-MP472341BQP
CSB-EP472341BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus MutS2 protein (mutS2), partial CSB-YP472342BQP
CSB-EP472342BQP
CSB-BP472342BQP
CSB-MP472342BQP
CSB-EP472342BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L20 (rplT) CSB-YP472343BQP
CSB-EP472343BQP
CSB-BP472343BQP
CSB-MP472343BQP
CSB-EP472343BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0756 membrane protein BCG9842_B0533 (BCG9842_B0533), partial CSB-YP472344BQP1
CSB-EP472344BQP1
CSB-BP472344BQP1
CSB-MP472344BQP1
CSB-EP472344BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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