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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acinetobacter baumannii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP472125AWN
CSB-EP472125AWN
CSB-BP472125AWN
CSB-MP472125AWN
CSB-EP472125AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Lon protease (lon), partial CSB-YP472126AWN
CSB-EP472126AWN
CSB-BP472126AWN
CSB-MP472126AWN
CSB-EP472126AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Urease subunit gamma (ureA) CSB-YP472127AWN
CSB-EP472127AWN
CSB-BP472127AWN
CSB-MP472127AWN
CSB-EP472127AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP472128AWN
CSB-EP472128AWN
CSB-BP472128AWN
CSB-MP472128AWN
CSB-EP472128AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP472129AWN
CSB-EP472129AWN
CSB-BP472129AWN
CSB-MP472129AWN
CSB-EP472129AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable malate:quinone oxidoreductase (mqo), partial CSB-YP472130AWN
CSB-EP472130AWN
CSB-BP472130AWN
CSB-MP472130AWN
CSB-EP472130AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP472131AWN
CSB-EP472131AWN
CSB-BP472131AWN
CSB-MP472131AWN
CSB-EP472131AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP472132AWN
CSB-EP472132AWN
CSB-BP472132AWN
CSB-MP472132AWN
CSB-EP472132AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Type III pantothenate kinase (coaX) CSB-YP472133AWN
CSB-EP472133AWN
CSB-BP472133AWN
CSB-MP472133AWN
CSB-EP472133AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP472134AWN
CSB-EP472134AWN
CSB-BP472134AWN
CSB-MP472134AWN
CSB-EP472134AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP472135AWN
CSB-EP472135AWN
CSB-BP472135AWN
CSB-MP472135AWN
CSB-EP472135AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Pantothenate synthetase (panC) CSB-YP472136AWN
CSB-EP472136AWN
CSB-BP472136AWN
CSB-MP472136AWN
CSB-EP472136AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Protein-export protein secB (secB) CSB-YP472137AWN
CSB-EP472137AWN
CSB-BP472137AWN
CSB-MP472137AWN
CSB-EP472137AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP472138AWN
CSB-EP472138AWN
CSB-BP472138AWN
CSB-MP472138AWN
CSB-EP472138AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Homoserine O-acetyltransferase (metX) CSB-YP472139AWN
CSB-EP472139AWN
CSB-BP472139AWN
CSB-MP472139AWN
CSB-EP472139AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP472140AWN1
CSB-EP472140AWN1
CSB-BP472140AWN1
CSB-MP472140AWN1
CSB-EP472140AWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosome maturation factor RimP (rimP) CSB-YP472141AWN
CSB-EP472141AWN
CSB-BP472141AWN
CSB-MP472141AWN
CSB-EP472141AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP472142AWN
CSB-EP472142AWN
CSB-BP472142AWN
CSB-MP472142AWN
CSB-EP472142AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP472143AWN
CSB-EP472143AWN
CSB-BP472143AWN
CSB-MP472143AWN
CSB-EP472143AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP472144AWN
CSB-EP472144AWN
CSB-BP472144AWN
CSB-MP472144AWN
CSB-EP472144AWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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