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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 ATP synthase epsilon chain (atpC) CSB-YP473386SWO
CSB-EP473386SWO
CSB-BP473386SWO
CSB-MP473386SWO
CSB-EP473386SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Cysteine--tRNA ligase (cysS) CSB-YP473387SWO
CSB-EP473387SWO
CSB-BP473387SWO
CSB-MP473387SWO
CSB-EP473387SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Tyrosine--tRNA ligase (tyrS) CSB-YP473388SWO
CSB-EP473388SWO
CSB-BP473388SWO
CSB-MP473388SWO
CSB-EP473388SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 S-ribosylhomocysteine lyase (luxS) CSB-YP473389SWO
CSB-EP473389SWO
CSB-BP473389SWO
CSB-MP473389SWO
CSB-EP473389SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 ATP synthase subunit c (atpE), partial CSB-YP473390SWO1
CSB-EP473390SWO1
CSB-BP473390SWO1
CSB-MP473390SWO1
CSB-EP473390SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Low affinity potassium transport system protein kup (kup), partial CSB-YP473391SWO
CSB-EP473391SWO
CSB-BP473391SWO
CSB-MP473391SWO
CSB-EP473391SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP473392SWO
CSB-EP473392SWO
CSB-BP473392SWO
CSB-MP473392SWO
CSB-EP473392SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 3-dehydroquinate dehydratase (aroD) CSB-YP473393SWO
CSB-EP473393SWO
CSB-BP473393SWO
CSB-MP473393SWO
CSB-EP473393SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 50S ribosomal protein L20 (rplT) CSB-YP473394SWO
CSB-EP473394SWO
CSB-BP473394SWO
CSB-MP473394SWO
CSB-EP473394SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP473395SWO
CSB-EP473395SWO
CSB-BP473395SWO
CSB-MP473395SWO
CSB-EP473395SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Der GTPase-activating protein YihI (yihI) CSB-YP473396SWO
CSB-EP473396SWO
CSB-BP473396SWO
CSB-MP473396SWO
CSB-EP473396SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Potassium-transporting ATPase B chain (kdpB), partial CSB-YP473397SWO1
CSB-EP473397SWO1
CSB-BP473397SWO1
CSB-MP473397SWO1
CSB-EP473397SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0249 protein ChbG (chbG) CSB-YP473398SWO
CSB-EP473398SWO
CSB-BP473398SWO
CSB-MP473398SWO
CSB-EP473398SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 L-fucose isomerase (fucI), partial CSB-YP473399SWO
CSB-EP473399SWO
CSB-BP473399SWO
CSB-MP473399SWO
CSB-EP473399SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein FdhE (fdhE) CSB-YP473400SWO
CSB-EP473400SWO
CSB-BP473400SWO
CSB-MP473400SWO
CSB-EP473400SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Rhamnulokinase (rhaB) CSB-YP473401SWO
CSB-EP473401SWO
CSB-BP473401SWO
CSB-MP473401SWO
CSB-EP473401SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein TolB (tolB) CSB-YP473402SWO
CSB-EP473402SWO
CSB-BP473402SWO
CSB-MP473402SWO
CSB-EP473402SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Formimidoylglutamase (hutG) CSB-YP473403SWO
CSB-EP473403SWO
CSB-BP473403SWO
CSB-MP473403SWO
CSB-EP473403SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Biotin synthase (bioB) CSB-YP473404SWO
CSB-EP473404SWO
CSB-BP473404SWO
CSB-MP473404SWO
CSB-EP473404SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Beta-hexosaminidase (nagZ) CSB-YP473405SWO
CSB-EP473405SWO
CSB-BP473405SWO
CSB-MP473405SWO
CSB-EP473405SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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