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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Vibrio fischeri Aspartate carbamoyltransferase (pyrB) CSB-YP474986VFC
CSB-EP474986VFC
CSB-BP474986VFC
CSB-MP474986VFC
CSB-EP474986VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP474987VFC
CSB-EP474987VFC
CSB-BP474987VFC
CSB-MP474987VFC
CSB-EP474987VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0325 protein VFMJ11_2099 (VFMJ11_2099) CSB-YP474988VFC
CSB-EP474988VFC
CSB-BP474988VFC
CSB-MP474988VFC
CSB-EP474988VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Chaperone protein DnaK (dnaK), partial CSB-YP474989VFC
CSB-EP474989VFC
CSB-BP474989VFC
CSB-MP474989VFC
CSB-EP474989VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S20 (rpsT) CSB-YP474990VFC
CSB-EP474990VFC
CSB-BP474990VFC
CSB-MP474990VFC
CSB-EP474990VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphoglucosamine mutase (glmM) CSB-YP474991VFC
CSB-EP474991VFC
CSB-BP474991VFC
CSB-MP474991VFC
CSB-EP474991VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Peptide chain release factor 3 (prfC), partial CSB-YP474992VFC
CSB-EP474992VFC
CSB-BP474992VFC
CSB-MP474992VFC
CSB-EP474992VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosome maturation factor RimM (rimM) CSB-YP474993VFC
CSB-EP474993VFC
CSB-BP474993VFC
CSB-MP474993VFC
CSB-EP474993VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP474994VFC
CSB-EP474994VFC
CSB-BP474994VFC
CSB-MP474994VFC
CSB-EP474994VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0149 protein VFMJ11_2207 (VFMJ11_2207) CSB-YP474995VFC
CSB-EP474995VFC
CSB-BP474995VFC
CSB-MP474995VFC
CSB-EP474995VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Homoserine kinase (thrB) CSB-YP474996VFC
CSB-EP474996VFC
CSB-BP474996VFC
CSB-MP474996VFC
CSB-EP474996VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Trp operon repressor homolog (trpR) CSB-YP474997VFC
CSB-EP474997VFC
CSB-BP474997VFC
CSB-MP474997VFC
CSB-EP474997VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0597 protein VFMJ11_0655 (VFMJ11_0655) CSB-YP474998VFC
CSB-EP474998VFC
CSB-BP474998VFC
CSB-MP474998VFC
CSB-EP474998VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri UPF0102 protein VFMJ11_2324 (VFMJ11_2324) CSB-YP474999VFC
CSB-EP474999VFC
CSB-BP474999VFC
CSB-MP474999VFC
CSB-EP474999VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Urease accessory protein UreG (ureG) CSB-YP475000VFC
CSB-EP475000VFC
CSB-BP475000VFC
CSB-MP475000VFC
CSB-EP475000VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Urease subunit alpha (ureC) CSB-YP475001VFC
CSB-EP475001VFC
CSB-BP475001VFC
CSB-MP475001VFC
CSB-EP475001VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP475002VFC
CSB-EP475002VFC
CSB-BP475002VFC
CSB-MP475002VFC
CSB-EP475002VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Na (+)-translocating NADH-quinone reductase subunit A CSB-YP475003VFC
CSB-EP475003VFC
CSB-BP475003VFC
CSB-MP475003VFC
CSB-EP475003VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP475004VFC
CSB-EP475004VFC
CSB-BP475004VFC
CSB-MP475004VFC
CSB-EP475004VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri DNA mismatch repair protein mutL (mutL), partial CSB-YP475005VFC
CSB-EP475005VFC
CSB-BP475005VFC
CSB-MP475005VFC
CSB-EP475005VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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