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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella enteritidis PT4 Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP473446SWO
CSB-EP473446SWO
CSB-BP473446SWO
CSB-MP473446SWO
CSB-EP473446SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 D-amino acid dehydrogenase small subunit (dadA) CSB-YP473447SWO
CSB-EP473447SWO
CSB-BP473447SWO
CSB-MP473447SWO
CSB-EP473447SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP473448SWO
CSB-EP473448SWO
CSB-BP473448SWO
CSB-MP473448SWO
CSB-EP473448SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP473449SWO
CSB-EP473449SWO
CSB-BP473449SWO
CSB-MP473449SWO
CSB-EP473449SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP473450SWO
CSB-EP473450SWO
CSB-BP473450SWO
CSB-MP473450SWO
CSB-EP473450SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glycogen synthase (glgA) CSB-YP473451SWO
CSB-EP473451SWO
CSB-BP473451SWO
CSB-MP473451SWO
CSB-EP473451SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Pantothenate synthetase (panC) CSB-YP473452SWO
CSB-EP473452SWO
CSB-BP473452SWO
CSB-MP473452SWO
CSB-EP473452SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP473453SWO
CSB-EP473453SWO
CSB-BP473453SWO
CSB-MP473453SWO
CSB-EP473453SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Outer membrane protein assembly factor yaeT (yaeT), partial CSB-YP473454SWO
CSB-EP473454SWO
CSB-BP473454SWO
CSB-MP473454SWO
CSB-EP473454SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Probable intracellular septation protein A (yciB), partial CSB-YP473455SWO1
CSB-EP473455SWO1
CSB-BP473455SWO1
CSB-MP473455SWO1
CSB-EP473455SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Chorismate synthase (aroC) CSB-YP473456SWO
CSB-EP473456SWO
CSB-BP473456SWO
CSB-MP473456SWO
CSB-EP473456SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Protein CysZ (cysZ), partial CSB-YP473457SWO1
CSB-EP473457SWO1
CSB-BP473457SWO1
CSB-MP473457SWO1
CSB-EP473457SWO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Nickel-responsive regulator (nikR) CSB-YP473458SWO
CSB-EP473458SWO
CSB-BP473458SWO
CSB-MP473458SWO
CSB-EP473458SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP473459SWO
CSB-EP473459SWO
CSB-BP473459SWO
CSB-MP473459SWO
CSB-EP473459SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP473460SWO
CSB-EP473460SWO
CSB-BP473460SWO
CSB-MP473460SWO
CSB-EP473460SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 UPF0214 protein yfeW (yfeW) CSB-YP473461SWO
CSB-EP473461SWO
CSB-BP473461SWO
CSB-MP473461SWO
CSB-EP473461SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 2,3-diketo-L-gulonate reductase (dlgD) CSB-YP473462SWO
CSB-EP473462SWO
CSB-BP473462SWO
CSB-MP473462SWO
CSB-EP473462SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Gamma-glutamyl phosphate reductase (proA) CSB-YP473463SWO
CSB-EP473463SWO
CSB-BP473463SWO
CSB-MP473463SWO
CSB-EP473463SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Histidine--tRNA ligase (hisS) CSB-YP473464SWO
CSB-EP473464SWO
CSB-BP473464SWO
CSB-MP473464SWO
CSB-EP473464SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Co-chaperone protein hscB (hscB) CSB-YP473465SWO
CSB-EP473465SWO
CSB-BP473465SWO
CSB-MP473465SWO
CSB-EP473465SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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