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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O157:H7 Copper homeostasis protein CutC (cutC) CSB-YP473786EOE
CSB-EP473786EOE
CSB-BP473786EOE
CSB-MP473786EOE
CSB-EP473786EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP473787EOE
CSB-EP473787EOE
CSB-BP473787EOE
CSB-MP473787EOE
CSB-EP473787EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Bifunctional protein HldE (hldE) CSB-YP473788EOE
CSB-EP473788EOE
CSB-BP473788EOE
CSB-MP473788EOE
CSB-EP473788EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S21 (rpsU) CSB-YP473789EOE
CSB-EP473789EOE
CSB-BP473789EOE
CSB-MP473789EOE
CSB-EP473789EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 D-cysteine desulfhydrase (dcyD) CSB-YP473790EOE
CSB-EP473790EOE
CSB-BP473790EOE
CSB-MP473790EOE
CSB-EP473790EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Threonine/serine transporter TdcC (tdcC), partial CSB-YP473791EOE
CSB-EP473791EOE
CSB-BP473791EOE
CSB-MP473791EOE
CSB-EP473791EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0306 protein yhbP (yhbP) CSB-YP473792EOE
CSB-EP473792EOE
CSB-BP473792EOE
CSB-MP473792EOE
CSB-EP473792EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Phosphoglucosamine mutase (glmM) CSB-YP473793EOE
CSB-EP473793EOE
CSB-BP473793EOE
CSB-MP473793EOE
CSB-EP473793EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP473794EOE
CSB-EP473794EOE
CSB-BP473794EOE
CSB-MP473794EOE
CSB-EP473794EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0145 protein ybjQ (ybjQ) CSB-YP473795EOE
CSB-EP473795EOE
CSB-BP473795EOE
CSB-MP473795EOE
CSB-EP473795EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP473796EOE1
CSB-EP473796EOE1
CSB-BP473796EOE1
CSB-MP473796EOE1
CSB-EP473796EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DNA-binding protein fis (fis) CSB-YP473797EOE
CSB-EP473797EOE
CSB-BP473797EOE
CSB-MP473797EOE
CSB-EP473797EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Large-conductance mechanosensitive channel (mscL), partial CSB-YP473798EOE1
CSB-EP473798EOE1
CSB-BP473798EOE1
CSB-MP473798EOE1
CSB-EP473798EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Serine--tRNA ligase (serS) CSB-YP473799EOE
CSB-EP473799EOE
CSB-BP473799EOE
CSB-MP473799EOE
CSB-EP473799EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cytidylate kinase (cmk) CSB-YP473800EOE
CSB-EP473800EOE
CSB-BP473800EOE
CSB-MP473800EOE
CSB-EP473800EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Chromosome partition protein MukB (mukB), partial CSB-YP473801EOE
CSB-EP473801EOE
CSB-BP473801EOE
CSB-MP473801EOE
CSB-EP473801EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Macrodomain Ter protein (matP) CSB-YP473802EOE
CSB-EP473802EOE
CSB-BP473802EOE
CSB-MP473802EOE
CSB-EP473802EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP473803EOE
CSB-EP473803EOE
CSB-BP473803EOE
CSB-MP473803EOE
CSB-EP473803EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L14 (rplN) CSB-YP473804EOE
CSB-EP473804EOE
CSB-BP473804EOE
CSB-MP473804EOE
CSB-EP473804EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L4 (rplD) CSB-YP473805EOE
CSB-EP473805EOE
CSB-BP473805EOE
CSB-MP473805EOE
CSB-EP473805EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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